Literature DB >> 1511138

Genetic transformation of Norway spruce (Picea abies (L.) Karst) using somatic embryo explants by microprojectile bombardment.

D Robertson1, A K Weissinger, R Ackley, S Glover, R R Sederoff.   

Abstract

Stable transformation of Norway spruce tissue has been obtained following bombardment of mature somatic embryos with pRT99gus, a plasmid that contains neo coding for NPTII, and gusA, coding for beta-glucuronidase, both fused to the CaMV 35S promoter. At least 8 lines have been stably transformed (over 15 months in culture) following bombardment and selection on kanamycin. Polymerase chain reaction analyses showed a high frequency of cotransformation of the gusA and neo genes. The frequency of coexpression of the selected and unselected markers was 100%. DNA/DNA hybridization of one transformed line provided conclusive evidence of stable integration and showed copy numbers of over 10 plasmid sequences per genome. None of the transformed lines has remained embryogenic.

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Year:  1992        PMID： 1511138     DOI： 10.1007/bf00040525

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  22 in total

1.  Genetic transformation of maize cells by particle bombardment.

Authors:  T M Klein; L Kornstein; J C Sanford; M E Fromm
Journal:  Plant Physiol       Date:  1989-09       Impact factor: 8.340

2.  Transgenic tobacco plants and their progeny derived by microprojectile bombardment of tobacco leaves.

Authors:  D T Tomes; A K Weissinger; M Ross; R Higgins; B J Drummond; S Schaaf; J Malone-Schoneberg; M Staebell; P Flynn; J Anderson
Journal:  Plant Mol Biol       Date:  1990-02       Impact factor: 4.076

3.  T-DNA presence and opine production in tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281.

Authors:  E E Hood; D H Clapham; I Ekberg; T Johannson
Journal:  Plant Mol Biol       Date:  1990-02       Impact factor: 4.076

4.  Extended Host Range of Agrobacterium tumefaciens in the Genus Pinus.

Authors:  A M Stomp; C Loopstra; W S Chilton; R R Sederoff; L W Moore
Journal:  Plant Physiol       Date:  1990-04       Impact factor: 8.340

5.  Abscisic acid-responsive sequences from the em gene of wheat.

Authors:  W R Marcotte; S H Russell; R S Quatrano
Journal:  Plant Cell       Date:  1989-10       Impact factor: 11.277

6.  beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors:  R A Jefferson; S M Burgess; D Hirsh
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

7.  Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Authors:  W. J. Gordon-Kamm; T. M. Spencer; M. L. Mangano; T. R. Adams; R. J. Daines; W. G. Start; J. V. O'Brien; S. A. Chambers; W. R. Adams; N. G. Willetts; T. B. Rice; C. J. Mackey; R. W. Krueger; A. P. Kausch; P. G. Lemaux
Journal:  Plant Cell       Date:  1990-07       Impact factor: 11.277

8.  Transient expression of the β-glucuronidase gene in embryogenic callus of Picea mariana following microprojection.

Authors:  L C Duchesne; P J Charest
Journal:  Plant Cell Rep       Date:  1991-07       Impact factor: 4.570

9.  Transient expression from microprojectile-mediated DNA transfer in pinus taeda.

Authors:  A M Stomp; A Weissinger; R R Sederoff
Journal:  Plant Cell Rep       Date:  1991-07       Impact factor: 4.570

10.  Bialaphos selection of stable transformants from maize cell culture.

Authors:  T M Spencer; W J Gordon-Kamm; R J Daines; W G Start; P G Lemaux
Journal:  Theor Appl Genet       Date:  1990-05       Impact factor: 5.699
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  8 in total

1.  Expression of the green fluorescent protein gene in conifer tissues.

Authors:  Lining Tian; Armand Séguin; Pierre J Charest
Journal:  Plant Cell Rep       Date:  1997-02       Impact factor: 4.570

2.  High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).

Authors:  A R Wenck; M Quinn; R W Whetten; G Pullman; R Sederoff
Journal:  Plant Mol Biol       Date:  1999-02       Impact factor: 4.076

3.  The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth.

Authors:  Heidi Tiimonen; Hely Häggman; Chung-Jui Tsai; Vincent Chiang; Tuija Aronen
Journal:  Plant Cell Rep       Date:  2007-03-13       Impact factor: 4.570

4.  A biolistic approach for the transfer and expression of a gusA. reporter gene in embryogenic cultures of Pinus radiata.

Authors:  C Walter; D R Smith; M B Connett; L Grace; D W White
Journal:  Plant Cell Rep       Date:  1994-12       Impact factor: 4.570

5.  The production of transgenic Scots pine (Pinus sylvestris L.) via the application of transformed pollen in controlled crossings.

Authors:  Tuija S Aronen; Teijo O Nikkanen; Hely M Häggman
Journal:  Transgenic Res       Date:  2003-06       Impact factor: 2.788

6.  Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment.

Authors:  V R Bommineni; R N Chibbar; R S Datla; E W Tsang
Journal:  Plant Cell Rep       Date:  1993-11       Impact factor: 4.570

Review 7.  Genetic transformation of conifers and its application in forest biotechnology.

Authors:  W Tang; R J Newton
Journal:  Plant Cell Rep       Date:  2003-06-24       Impact factor: 4.570

8.  Phalaenopsis LEAFY COTYLEDON1-Induced Somatic Embryonic Structures Are Morphologically Distinct From Protocorm-Like Bodies.

Authors:  Jhun-Chen Chen; Chii-Gong Tong; Hsiang-Yin Lin; Su-Chiung Fang
Journal:  Front Plant Sci       Date:  2019-11-29       Impact factor: 5.753
  8 in total

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