Literature DB >> 210143

Purification of hepatitis A virus from human feces.

S A Locarnini, A G Coulepis, A A Ferris, N I Lehmann, I D Gust.   

Abstract

Hepatitis A virus was purified fecal specimens obtained from 2 patients with naturally acquired hepatitis A. The purification procedure involved differential centrifugation, organic solvent extraction, agarose gel filtration, ion-exchange chromatography, and isopycnic ultracentrifugation in cesium chloride. Using immune electron microscopy and discontinuous SDS-PAGE, this procedure was found to be effective in removing extraneous material from hepatitis A virus. There was significant recovery of virus as judged by immune electron microscopy and solid-phase radioimmunoassay. Using this protocol, it has been possible to obtain virus preparations of sufficient purity and high enough titer to enable biochemical studies to proceed.

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Year:  1978        PMID： 210143     DOI： 10.1159/000148992

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  10 in total

Review 1.  Diagnosis of hepatitis a virus infection: a molecular approach.

Authors:  Omana V Nainan; Guoliang Xia; Gilberto Vaughan; Harold S Margolis
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

2.  Protease digestion of hepatitis A virus: disparate effects on capsid proteins, antigenicity, and infectivity.

Authors:  S M Lemon; E Amphlett; D Sangar
Journal:  J Virol       Date:  1991-10       Impact factor: 5.103

3.  Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.

Authors:  A G Coulepis; M F Veale; A MacGregor; M Kornitschuk; I D Gust
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

4.  Evidence that the genome of hepatitis A virus consists of single-stranded RNA.

Authors:  A G Coulepis; G A Tannock; S A Locarnini; I D Gust
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103

5.  Monoclonal antibodies against hepatitis A virus.

Authors:  A MacGregor; M Kornitschuk; J G Hurrell; N I Lehmann; A G Coulepis; S A Locarnini; I D Gust
Journal:  J Clin Microbiol       Date:  1983-11       Impact factor: 5.948

6.  Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M.

Authors:  S A Locarnini; A G Coulepis; A M Stratton; J Kaldor; I D Gust
Journal:  J Clin Microbiol       Date:  1979-04       Impact factor: 5.948

7.  Iodination of hepatitis A virus reveals a fourth structural polypeptide.

Authors:  A G Coulepis; S A Locarnini; I D Gust
Journal:  J Virol       Date:  1980-08       Impact factor: 5.103

8.  Coproantibodies in hepatitis A: detection by enzyme-linked immunosorbent assay and immune electron microscopy.

Authors:  S A Locarnini; A G Coulepis; J Kaldor; I D Gust
Journal:  J Clin Microbiol       Date:  1980-06       Impact factor: 5.948

9.  Purification of infectious rubella virus by gel filtration on sepharose 2B compared to gradient centrifugation in sucrose, sodium metrizoate and metrizamide.

Authors:  M Trudel; F Marchessault; P Payment
Journal:  J Virol Methods       Date:  1981-02       Impact factor: 2.014

10.  Restricted replication of human hepatitis A virus in cell culture: intracellular biochemical studies.

Authors:  S A Locarnini; A G Coulepis; E G Westaway; I D Gust
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103
  10 in total

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