Literature DB >> 20978109

High-efficiency siRNA-based gene knockdown in human embryonic stem cells.

Yinghong Ma1, Jianyu Jin, Chunsheng Dong, Ee-Chun Cheng, Haifan Lin, Yingqun Huang, Caihong Qiu.   

Abstract

Loss-of-function studies in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) via nonviral approaches have been largely unsuccessful. Here we report a simple and cost-effective method for high-efficiency delivery of plasmids and siRNAs into hESCs and iPSCs. Using this method for siRNA delivery, we achieve >90% reduction in the expression of the stem cell factors Oct4 and Lin28, and observe cell morphological and staining pattern changes, characteristics of hESC differentiation, as a result of Oct4 knockdown.

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Year:  2010        PMID： 20978109      PMCID： PMC2995416          DOI： 10.1261/rna.2350710

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  13 in total

1.  Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.

Authors:  Shuyuan Yao; Shuibing Chen; Julie Clark; Ergeng Hao; Gillian M Beattie; Alberto Hayek; Sheng Ding
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-21       Impact factor: 11.205

2.  Method to enhance transfection efficiency of cell lines and placental fibroblasts.

Authors:  M Zhang; S Guller; Y Huang
Journal:  Placenta       Date:  2007-03-12       Impact factor: 3.481

Review 3.  Regulation of pluripotency and reprogramming by transcription factors.

Authors:  Duanqing Pei
Journal:  J Biol Chem       Date:  2008-09-26       Impact factor: 5.157

4.  ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells.

Authors:  David A Claassen; Michelle M Desler; Angie Rizzino
Journal:  Mol Reprod Dev       Date:  2009-08       Impact factor: 2.609

5.  High-efficiency RNA interference in human embryonic stem cells.

Authors:  Holm Zaehres; M William Lensch; Laurence Daheron; Sheila A Stewart; Joseph Itskovitz-Eldor; George Q Daley
Journal:  Stem Cells       Date:  2005-03       Impact factor: 6.277

6.  Induced pluripotent stem cell lines derived from human somatic cells.

Authors:  Junying Yu; Maxim A Vodyanik; Kim Smuga-Otto; Jessica Antosiewicz-Bourget; Jennifer L Frane; Shulan Tian; Jeff Nie; Gudrun A Jonsdottir; Victor Ruotti; Ron Stewart; Igor I Slukvin; James A Thomson
Journal:  Science       Date:  2007-11-20       Impact factor: 47.728

7.  Embryonic stem cell lines derived from human blastocysts.

Authors:  J A Thomson; J Itskovitz-Eldor; S S Shapiro; M A Waknitz; J J Swiergiel; V S Marshall; J M Jones
Journal:  Science       Date:  1998-11-06       Impact factor: 47.728

8.  Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

Authors:  Kazutoshi Takahashi; Koji Tanabe; Mari Ohnuki; Megumi Narita; Tomoko Ichisaka; Kiichiro Tomoda; Shinya Yamanaka
Journal:  Cell       Date:  2007-11-30       Impact factor: 41.582

9.  Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.

Authors:  Caihong Qiu; Yinghong Ma; Jianquan Wang; Shuping Peng; Yingqun Huang
Journal:  Nucleic Acids Res       Date:  2009-12-04       Impact factor: 16.971

10.  Histone H2a mRNA interacts with Lin28 and contains a Lin28-dependent posttranscriptional regulatory element.

Authors:  Bingsen Xu; Yingqun Huang
Journal:  Nucleic Acids Res       Date:  2009-05-13       Impact factor: 16.971
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  17 in total

1.  Directing neuronal differentiation of primary neural progenitor cells by gene knockdown approach.

Authors:  Wei Ching Low; Winifred Wing Yiu Yau; Lawrence W Stanton; Guillaume Marcy; Eyleen Goh; Sing Yian Chew
Journal:  DNA Cell Biol       Date:  2012-02-17       Impact factor: 3.311

2.  Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs.

Authors:  Estela Cruvinel; Tara Budinetz; Noelle Germain; Stormy Chamberlain; Marc Lalande; Kristen Martins-Taylor
Journal:  Hum Mol Genet       Date:  2014-04-23       Impact factor: 6.150

Review 3.  Endocytosis in gene therapy with non-viral vectors.

Authors:  Aritz Perez Ruiz de Garibay
Journal:  Wien Med Wochenschr       Date:  2016-05-03

4.  Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes.

Authors:  Jianyu Jin; Wei Jing; Xin-Xiang Lei; Chen Feng; Shuping Peng; Kathleen Boris-Lawrie; Yingqun Huang
Journal:  Nucleic Acids Res       Date:  2011-01-18       Impact factor: 16.971

5.  High glutathione and glutathione peroxidase-2 levels mediate cell-type-specific DNA damage protection in human induced pluripotent stem cells.

Authors:  Benjamin Dannenmann; Simon Lehle; Dominic G Hildebrand; Ayline Kübler; Paula Grondona; Vera Schmid; Katharina Holzer; Mirjam Fröschl; Frank Essmann; Oliver Rothfuss; Klaus Schulze-Osthoff
Journal:  Stem Cell Reports       Date:  2015-04-30       Impact factor: 7.765

6.  Sprouty genes regulate proliferation and survival of human embryonic stem cells.

Authors:  Hady Felfly; Ophir D Klein
Journal:  Sci Rep       Date:  2013       Impact factor: 4.379

7.  Fast and Efficient Transfection of Mouse Embryonic Stem Cells Using Non-Viral Reagents.

Authors:  Christoffer Tamm; Sandeep Kadekar; Sara Pijuan-Galitó; Cecilia Annerén
Journal:  Stem Cell Rev Rep       Date:  2016-10       Impact factor: 5.739

8.  Loss of RAD9B impairs early neural development and contributes to the risk for human spina bifida.

Authors:  Xuanye Cao; Tian Tian; John W Steele; Robert M Cabrera; Vanessa Aguiar-Pulido; Shruti Wadhwa; Nikitha Bhavani; Patrick Bi; Nick H Gargurevich; Ethan N Hoffman; Chun-Quan Cai; Nicholas J Marini; Wei Yang; Gary M Shaw; Margaret E Ross; Richard H Finnell; Yunping Lei
Journal:  Hum Mutat       Date:  2020-01-16       Impact factor: 4.700

9.  Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency.

Authors:  Takenobu Nii; Hiroshi Kohara; Tomotoshi Marumoto; Tetsushi Sakuma; Takashi Yamamoto; Kenzaburo Tani
Journal:  Biores Open Access       Date:  2016-05-01

10.  Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells.

Authors:  Hongran Wang; Xiaohong Wang; Xueping Xu; Michael Kyba; Austin J Cooney
Journal:  J Biol Chem       Date:  2016-01-14       Impact factor: 5.157
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