Literature DB >> 20959798

A model for harmonizing flow cytometry in clinical trials.

Holden T Maecker1, J Philip McCoy, Michael Amos, John Elliott, Adolfas Gaigalas, Lili Wang, Richard Aranda, Jacques Banchereau, Chris Boshoff, Jonathan Braun, Yael Korin, Elaine Reed, Judy Cho, David Hafler, Mark Davis, C Garrison Fathman, William Robinson, Thomas Denny, Kent Weinhold, Bela Desai, Betty Diamond, Peter Gregersen, Paola Di Meglio, Paola DiMeglio, Frank O Nestle, Frank Nestle, Mark Peakman, Federica Villanova, Federica Villnova, John Ferbas, Elizabeth Field, Aaron Kantor, Thomas Kawabata, Wendy Komocsar, Michael Lotze, Jerry Nepom, Hans Ochs, Raegan O'Lone, Deborah Phippard, Scott Plevy, Stephen Rich, Mario Roederer, Dan Rotrosen, Jung-Hua Yeh.   

Abstract

Complexities in sample handling, instrument setup and data analysis are barriers to the effective use of flow cytometry to monitor immunological parameters in clinical trials. The novel use of a central laboratory may help mitigate these issues.

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Year:  2010        PMID： 20959798      PMCID： PMC3400260          DOI： 10.1038/ni1110-975

Source DB:  PubMed          Journal:  Nat Immunol        ISSN： 1529-2908            Impact factor:   25.606


  26 in total

1.  Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.

Authors:  M Roederer
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2.  Detection of antigen-specific T cells by cytokine flow cytometry: the use of whole blood may underestimate frequencies.

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3.  Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation.

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Authors:  Stephen P Perfetto; Pratip K Chattopadhyay; Mario Roederer
Journal:  Nat Rev Immunol       Date:  2004-08       Impact factor: 53.106

5.  Analysis of T cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry.

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7.  "MIATA"-minimal information about T cell assays.

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10.  Performance of plate-based cytokine flow cytometry with automated data analysis.

Authors:  Maria A Suni; Holli S Dunn; Patricia L Orr; Rian de Laat; Elizabeth Sinclair; Smita A Ghanekar; Barry M Bredt; John F Dunne; Vernon C Maino; Holden T Maecker
Journal:  BMC Immunol       Date:  2003-09-02       Impact factor: 3.615
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Review 5.  The Antibody-Secreting Cell Response to Infection: Kinetics and Clinical Applications.

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8.  Optimization of a whole blood phenotyping assay for enumeration of peripheral blood leukocyte populations in multicenter clinical trials.

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Review 9.  A review of the application of inflammatory biomarkers in epidemiologic cancer research.

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10.  Setting objective thresholds for rare event detection in flow cytometry.

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