Literature DB >> 1401946

Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation.

M A Romeu1, M Mestre, L González, A Valls, J Verdaguer, M Corominas, J Bas, E Massip, E Buendia.   

Abstract

In the present report we have assessed the extent to which Ficoll-Paque separation and cryopreservation of mononuclear cells alter the measurement of lymphocyte subsets by flow cytometry. Standard Ficoll-Paque separation increased the percentage of CD4+, CD19+ and CD4+CD45RA+ cells, as well as decreasing that of CD8+, and CD4+CD29+ cells, compared to the fresh whole blood lysis technique. Moreover, cryopreservation caused a depletion of CD4+ p80+ cells, but normal whole blood values were restored following a short incubation.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1401946     DOI： 10.1016/0022-1759(92)90206-9

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  15 in total

1.  Effects of delays in peripheral blood processing, including cryopreservation, on detection of CD31 expression on naive CD4 T cells.

Authors:  Jeanette Higgins; Julia A Metcalf; Randy A Stevens; Michael Baseler; Michael Proschan; H Clifford Lane; Irini Sereti
Journal:  Clin Vaccine Immunol       Date:  2008-04-30

2.  Comparison of the effects of Ficoll-Hypaque separation and whole blood lysis on results of immunophenotypic analysis of blood and bone marrow samples from patients with hematologic malignancies.

Authors:  K R Tamul; J L Schmitz; K Kane; J D Folds
Journal:  Clin Diagn Lab Immunol       Date:  1995-05

3.  Lymphocyte subpopulations in newborns.

Authors:  M Raes; J L Rummens
Journal:  Eur J Pediatr       Date:  1995-05       Impact factor: 3.183

4.  Treatment with methylprednisolone in relapses of multiple sclerosis patients: immunological evidence of immediate and short-term but not long-lasting effects.

Authors:  E M Martínez-Cáceres; M A Barrau; L Brieva; C Espejo; N Barberà; X Montalban
Journal:  Clin Exp Immunol       Date:  2002-01       Impact factor: 4.330

5.  Distribution of lymphocyte surface antigens in healthy neonates.

Authors:  U Kontny; C Barrachina; P Habermehl; W Mannhardt; F Zepp; O Schofer
Journal:  Eur J Pediatr       Date:  1994-04       Impact factor: 3.183

6.  Preservation of lymphocyte immunophenotype and proliferative responses in cryopreserved peripheral blood mononuclear cells from human immunodeficiency virus type 1-infected donors: implications for multicenter clinical trials. The ACTG Immunology Advanced Technology Laboratories.

Authors:  K A Reimann; M Chernoff; C L Wilkening; C E Nickerson; A L Landay
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

7.  Quality monitoring of HIV-1-infected and uninfected peripheral blood mononuclear cell samples in a resource-limited setting.

Authors:  Robert E Olemukan; Leigh Anne Eller; Benson J Ouma; Ben Etonu; Simon Erima; Prossy Naluyima; Denis Kyabaggu; Josephine H Cox; Johan K Sandberg; Fred Wabwire-Mangen; Nelson L Michael; Merlin L Robb; Mark S de Souza; Michael A Eller
Journal:  Clin Vaccine Immunol       Date:  2010-03-03

8.  Patients with inflammatory bowel disease (IBD) reveal increased induction capacity of intracellular interferon-gamma (IFN-gamma) in peripheral CD8+ lymphocytes co-cultured with intestinal epithelial cells.

Authors:  G Bisping; N Lügering; S Lütke-Brintrup; H G Pauels; G Schürmann; W Domschke; T Kucharzik
Journal:  Clin Exp Immunol       Date:  2001-01       Impact factor: 4.330

9.  Increased circulating myeloid-derived suppressor cells correlate with clinical cancer stage, metastatic tumor burden, and doxorubicin-cyclophosphamide chemotherapy.

Authors:  C Marcela Diaz-Montero; Mohamed Labib Salem; Michael I Nishimura; Elizabeth Garrett-Mayer; David J Cole; Alberto J Montero
Journal:  Cancer Immunol Immunother       Date:  2008-04-30       Impact factor: 6.968

10.  Loss of T cell responses following long-term cryopreservation.

Authors:  Rachel E Owen; Elizabeth Sinclair; Brinda Emu; John W Heitman; Dale F Hirschkorn; C Lorrie Epling; Qi Xuan Tan; Brian Custer; Jeffery M Harris; Mark A Jacobson; Joseph M McCune; Jeffery N Martin; Frederick M Hecht; Steven G Deeks; Philip J Norris
Journal:  J Immunol Methods       Date:  2007-08-08       Impact factor: 2.303
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.