Literature DB >> 22343568

Standardizing immunophenotyping for the Human Immunology Project.

Holden T Maecker1, J Philip McCoy, Robert Nussenblatt.   

Abstract

The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.

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Year:  2012        PMID: 22343568      PMCID: PMC3409649          DOI: 10.1038/nri3158

Source DB:  PubMed          Journal:  Nat Rev Immunol        ISSN: 1474-1733            Impact factor:   53.106


  57 in total

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Journal:  Science       Date:  2002-10-18       Impact factor: 47.728

2.  HIV-specific CD8+ T cell proliferation is coupled to perforin expression and is maintained in nonprogressors.

Authors:  Stephen A Migueles; Alisha C Laborico; W Lesley Shupert; M Shirin Sabbaghian; Ronald Rabin; Claire W Hallahan; Debbie Van Baarle; Stefan Kostense; Frank Miedema; Mary McLaughlin; Linda Ehler; Julia Metcalf; Shuying Liu; Mark Connors
Journal:  Nat Immunol       Date:  2002-10-07       Impact factor: 25.606

3.  Small (CD14+/CD16+) monocytes and regular monocytes in human blood.

Authors:  H W Ziegler-Heitbrock; M Ströbel; G Fingerle; T Schlunck; A Pforte; M Blumenstein; J G Haas
Journal:  Pathobiology       Date:  1991       Impact factor: 4.342

4.  Variables affecting T-lymphocyte subsets in a volunteer blood donor population.

Authors:  J D Lifson; S L Finch; D T Sasaki; E G Engleman
Journal:  Clin Immunol Immunopathol       Date:  1985-08

Review 5.  Flow cytometric analysis of lymphomas and acute leukemias.

Authors:  R E Duque
Journal:  Ann N Y Acad Sci       Date:  1993-03-20       Impact factor: 5.691

6.  Immune pathogenesis of AIDS and related syndromes.

Authors:  J L Fahey; J Giorgi; O Martínez-Maza; R Detels; R Mitsuyasu; J Taylor
Journal:  Ann Inst Pasteur Immunol       Date:  1987 Mar-Apr

7.  Expression of activation antigens, HLA-DR and CD38, on CD8 lymphocytes during HIV-1 infection.

Authors:  L Kestens; G Vanham; P Gigase; G Young; I Hannet; F Vanlangendonck; F Hulstaert; B A Bach
Journal:  AIDS       Date:  1992-08       Impact factor: 4.177

8.  Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry.

Authors:  L J Picker; M K Singh; Z Zdraveski; J R Treer; S L Waldrop; P R Bergstresser; V C Maino
Journal:  Blood       Date:  1995-08-15       Impact factor: 22.113

9.  Analysis of somatic mutation in five B cell subsets of human tonsil.

Authors:  V Pascual; Y J Liu; A Magalski; O de Bouteiller; J Banchereau; J D Capra
Journal:  J Exp Med       Date:  1994-07-01       Impact factor: 14.307

10.  Performance of plate-based cytokine flow cytometry with automated data analysis.

Authors:  Maria A Suni; Holli S Dunn; Patricia L Orr; Rian de Laat; Elizabeth Sinclair; Smita A Ghanekar; Barry M Bredt; John F Dunne; Vernon C Maino; Holden T Maecker
Journal:  BMC Immunol       Date:  2003-09-02       Impact factor: 3.615

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  376 in total

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Review 2.  Telomeres and immune competency.

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Journal:  Curr Opin Immunol       Date:  2012-05-22       Impact factor: 7.486

Review 3.  Systems immunogenetics of vaccines.

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Journal:  Semin Immunol       Date:  2013-07-22       Impact factor: 11.130

Review 4.  Targeting memory T cells in type 1 diabetes.

Authors:  Mario R Ehlers; Mark R Rigby
Journal:  Curr Diab Rep       Date:  2015-11       Impact factor: 4.810

5.  An integrated framework using high-dimensional mass cytometry and fluorescent flow cytometry identifies discrete B cell subsets in patients with red meat allergy.

Authors:  Kelly M Cox; Scott P Commins; Brian J Capaldo; Lisa J Workman; Thomas A E Platts-Mills; El-Ad D Amir; Josephine A Lannigan; Alexander J Schuyler; Loren D Erickson
Journal:  Clin Exp Allergy       Date:  2019-01-08       Impact factor: 5.018

Review 6.  Systems immunology of human malaria.

Authors:  Tuan M Tran; Babru Samal; Ewen Kirkness; Peter D Crompton
Journal:  Trends Parasitol       Date:  2012-05-15

7.  Regulatory B cells promote graft-versus-host disease prevention and maintain graft-versus-leukemia activity following allogeneic bone marrow transplantation.

Authors:  Yue Hu; Gan-Lin He; Xiang-Yu Zhao; Xiao-Su Zhao; Yu Wang; Lan-Ping Xu; Xiao-Hui Zhang; Xue-Zhong Yu; Kai-Yan Liu; Ying-Jun Chang; Xiao-Jun Huang
Journal:  Oncoimmunology       Date:  2017-02-06       Impact factor: 8.110

8.  Cryptococcosis-Associated Immune Reconstitution Inflammatory Syndrome Is Associated With Dysregulation of IL-7/IL-7 Receptor Signaling Pathway in T Cells and Monocyte Activation.

Authors:  Ngomu Akeem Akilimali; Daniel M Muema; Charles Specht; Christina C Chang; Mahomed-Yunus S Moosa; Stuart M Levitz; Sharon R Lewin; Martyn A French; Thumbi Ndungʼu
Journal:  J Acquir Immune Defic Syndr       Date:  2019-04-15       Impact factor: 3.731

9.  High standards for high dimensional investigations.

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Journal:  Cytometry A       Date:  2016-09-23       Impact factor: 4.355

Review 10.  Pushing the frontiers of T-cell vaccines: accurate measurement of human T-cell responses.

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Journal:  Expert Rev Vaccines       Date:  2012-12       Impact factor: 5.217

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