| Literature DB >> 22343568 |
Holden T Maecker1, J Philip McCoy, Robert Nussenblatt.
Abstract
The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.Entities:
Mesh:
Year: 2012 PMID: 22343568 PMCID: PMC3409649 DOI: 10.1038/nri3158
Source DB: PubMed Journal: Nat Rev Immunol ISSN: 1474-1733 Impact factor: 53.106