ETHNOPHARMACOLOGICAL IMPORTANCE: Many species of plants in the Brazilian cerrado (savanna) are widely used in ethnomedicine. However, the safety and effectiveness of medicinal plants used in communities with little or no access to manufactured drugs should be evaluated. AIM OF THE STUDY: Evaluate the antimicrobial and cytotoxic activities of extracts from eight plant species, obtained using Brazilian cachaça as the extractor liquid. MATERIALS AND METHODS: The extracts were tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida parapsilosis, promastigote forms of Leishmania amazonensis, and poliovirus. In addition, cytotoxic activity was assayed in Vero cells and in human erythrocytes. RESULTS: The plant species Curatella americana, Sclerolobium aureum, and Plathymenia reticulata showed the best activity against yeasts, especially the crude extract of C. americana and its ethyl-acetate fraction. Kielmeyera lathrophyton showed a minimum inhibitory concentration of 250 μg/ml against S. aureus, and was inactive against gram-negative bacteria. The extract obtained from Annona coriacea showed the best activity against the promastigote forms of Leishmania amazonensis (IC(50)=175 μg/ml). Only C. americana showed potential for antipoliovirus activity. The concentrations of the crude extracts that showed toxicity to VERO cells had CC(50) between 31 and 470 μg/ml, and the lyophilized Brazilian cachaça showed a CC(50) of 307 μg/ml. None of the extracts showed toxicity against human erythrocytes. CONCLUSIONS: Among the plant species studied, C. americana proved to be effective against microorganisms, especially as an antifungal. The results will help in the search for alternative drugs to be used in pharmacotherapy, and will contribute to establish safe and effective use of phytomedicines in the treatment of infectious diseases. Copyright Â
ETHNOPHARMACOLOGICAL IMPORTANCE: Many species of plants in the Brazilian cerrado (savanna) are widely used in ethnomedicine. However, the safety and effectiveness of medicinal plants used in communities with little or no access to manufactured drugs should be evaluated. AIM OF THE STUDY: Evaluate the antimicrobial and cytotoxic activities of extracts from eight plant species, obtained using Brazilian cachaça as the extractor liquid. MATERIALS AND METHODS: The extracts were tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Candida parapsilosis, promastigote forms of Leishmania amazonensis, and poliovirus. In addition, cytotoxic activity was assayed in Vero cells and in human erythrocytes. RESULTS: The plant species Curatella americana, Sclerolobium aureum, and Plathymenia reticulata showed the best activity against yeasts, especially the crude extract of C. americana and its ethyl-acetate fraction. Kielmeyera lathrophyton showed a minimum inhibitory concentration of 250 μg/ml against S. aureus, and was inactive against gram-negative bacteria. The extract obtained from Annona coriacea showed the best activity against the promastigote forms of Leishmania amazonensis (IC(50)=175 μg/ml). Only C. americana showed potential for antipoliovirus activity. The concentrations of the crude extracts that showed toxicity to VERO cells had CC(50) between 31 and 470 μg/ml, and the lyophilized Brazilian cachaça showed a CC(50) of 307 μg/ml. None of the extracts showed toxicity against human erythrocytes. CONCLUSIONS: Among the plant species studied, C. americana proved to be effective against microorganisms, especially as an antifungal. The results will help in the search for alternative drugs to be used in pharmacotherapy, and will contribute to establish safe and effective use of phytomedicines in the treatment of infectious diseases. Copyright Â
Authors: Silvia T Elias; Carolina C S Macedo; Luiz A Simeoni; Dâmaris Silveira; Pérola O Magalhães; Adriana Lofrano-Porto; Ricardo D Coletta; Francisco A R Neves; Eliete N S Guerra Journal: Cell Cycle Date: 2016 Impact factor: 4.534
Authors: Farrapo Nicole M; Silva Gleidy Aa; Costa Karine N; Silva Magali G; Cogo José C; Belo Cháriston A Dal; Santos Márcio G Dos; Groppo Francisco C; Oshima-Franco Yoko Journal: J Venom Res Date: 2011-12-28
Authors: Maria Suênia P Silva; Deysiane O Brandão; Thiago P Chaves; Amaro L N Formiga Filho; Edja Maria M de B Costa; Vanda L Santos; Ana Cláudia D Medeiros Journal: Evid Based Complement Alternat Med Date: 2012-06-06 Impact factor: 2.629
Authors: Lia de Barros Leite Albuquerque; Cháriston André Dal Belo; Marcio Galdino dos Santos; Patricia Santos Lopes; Marli Gerenutti; Yoko Oshima-Franco Journal: Biomed Res Int Date: 2013-12-22 Impact factor: 3.411
Authors: Rafael Henrique Oliveira Lopes; Luis Fernando Benitez Macorini; Katia Ávila Antunes; Priscilla Pereira de Toledo Espindola; Tamaeh Monteiro Alfredo; Paola Dos Santos da Rocha; Zefa Valdivina Pereira; Edson Lucas Dos Santos; Kely de Picoli Souza Journal: Oxid Med Cell Longev Date: 2016-05-09 Impact factor: 6.543
Authors: Zulane Lima Sousa; Fernando Faustino de Oliveira; Aline Oliveira da Conceição; Luiz Alberto Mattos Silva; Maria Helena Rossi; Juliana da Silva Santos; João Luciano Andrioli Journal: Ann Clin Microbiol Antimicrob Date: 2012-07-28 Impact factor: 3.944
Authors: Cleyton Eduardo Mendes de Toledo; Patrícia Regina Santos; João Carlos Palazzo de Mello; Benedito Prado Dias Filho; Celso Vataru Nakamura; Tânia Ueda-Nakamura Journal: Evid Based Complement Alternat Med Date: 2015-08-11 Impact factor: 2.629
Authors: Mariana L de Mesquita; Waleska F Leão; Magda R A Ferreira; José E de Paula; Laila S Espindola; Luiz A L Soares Journal: Pharmacogn Mag Date: 2015-10 Impact factor: 1.085