Literature DB >> 20946913

Effect of ionic liquids on the structure, stability and activity of two related α-amylases.

Bahareh Dabirmanesh1, Sara Daneshjou, Abbas Akhavan Sepahi, Bijan Ranjbar, Ramazan Ali Khavari-Nejad, Pooria Gill, Akbar Heydari, Khosro Khajeh.   

Abstract

Ionic liquids are recognized as green solvents for carbohydrates dissolution. However, only a limited number of studies have been carried out to investigate their effect on carbohydrate hydrolyzing enzymes. We have investigated the influence of two water miscible ionic liquids on the activity, stability and structure of two related α-amylases from Bacillus amyloliquefaciens and Bacillus lichiniformis. Upon changes in ionic liquids concentrations, both enzymes activity and stability were reduced. Associated thermodynamic and conformational changes were observed using differential scanning calorimetry and fluorescence techniques. Thermal denaturation was accompanied by aggregation in both aqueous buffer and [BMIm][Cl] but [HMIm][Cl] significantly suppressed aggregation.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20946913     DOI： 10.1016/j.ijbiomac.2010.10.001

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN： 0141-8130            Impact factor:   6.953


  6 in total

1.  Purification and biochemical characterization of an acidophilic amylase from a newly isolated Bacillus sp. DR90.

Authors:  Ahmad Asoodeh; Ashraf Alemi; Akbar Heydari; Jafar Akbari
Journal:  Extremophiles       Date:  2013-02-21       Impact factor: 2.395

2.  Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II.

Authors:  Azadeh Fallah-Bagheri; Ali Akbar Saboury; Leila Ma'mani; Mohammad Taghizadeh; Reza Khodarahmi; Samira Ranjbar; Mousa Bohlooli; Abbas Shafiee; Alireza Foroumadi; Nader Sheibani; Ali Akbar Moosavi-Movahedi
Journal:  Int J Biol Macromol       Date:  2012-07-22       Impact factor: 6.953

Review 3.  Effect of water and ionic liquids on biomolecules.

Authors:  Debasis Saha; Arnab Mukherjee
Journal:  Biophys Rev       Date:  2018-02-08

4.  Properties of an ionic liquid-tolerant Bacillus amyloliquefaciens CMW1 and its extracellular protease.

Authors:  Atsushi Kurata; Humiya Senoo; Yasuyuki Ikeda; Hideaki Kaida; Chiaki Matsuhara; Noriaki Kishimoto
Journal:  Extremophiles       Date:  2016-05-03       Impact factor: 2.395

Review 5.  Aqueous ionic liquids in comparison with standard co-solutes : Differences and common principles in their interaction with protein and DNA structures.

Authors:  Ewa Anna Oprzeska-Zingrebe; Jens Smiatek
Journal:  Biophys Rev       Date:  2018-04-02

6.  Notable Stabilization of α-Chymotrypsin by the Protic Ionic Additive, [ch][dhp]: Calorimetric Evidence for a Fine Enthalpy/Entropy Balance.

Authors:  Sophio Uchaneishvili; Maya Makharadze; Mikhael Shushanyan; Rudi van Eldik; Dimitri E Khoshtariya
Journal:  Int Sch Res Notices       Date:  2014-09-07
  6 in total

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