| Literature DB >> 22829053 |
Azadeh Fallah-Bagheri1, Ali Akbar Saboury, Leila Ma'mani, Mohammad Taghizadeh, Reza Khodarahmi, Samira Ranjbar, Mousa Bohlooli, Abbas Shafiee, Alireza Foroumadi, Nader Sheibani, Ali Akbar Moosavi-Movahedi.
Abstract
Silica nanoparticle supported imidazolium ionic liquid [SNImIL] was synthesized and utilized as a biocompatible additive for studying the thermal reversibility of human carbonic anhydrase II (HCA II). For this purpose, we prepared additive by modification of nanoparticles through the grafting of ionic liquids on the surface of nanoparticles (SNImIL). The SNImIL were fully characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and thermo gravimetric analysis. The characterization of HCA II was investigated by various techniques including UV-vis and ANS fluorescence spectrophotometry, differential scanning calorimetry, and docking study. SNImIL induced disaggregation, enhanced protein stability and increased thermal reversibility of HCA II by up to 42% at pH 7.75.Entities:
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Year: 2012 PMID： 22829053 PMCID： PMC3677219 DOI： 10.1016/j.ijbiomac.2012.07.023
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953