OBJECTIVE: To provide recommendations for design and analysis of studies using urine specimens to evaluate renal function or mercury excretion in children. METHODS: An analysis of mercury, albumin, γ-glutamyl transpeptidase (γ-GT) and N-acetyl-β-D-glucosaminidase (NAG) concentrations was carried out. RESULTS: Mercury concentration and creatinine-corrected renal markers were higher in daytime compared with overnight samples. Excretion rates increased with urinary flow rate. γ-GT and NAG concentrations decreased with storage time at -20°C. Differences by age, sex and race were noted. CONCLUSIONS: We recommend use of these creatinine-corrected markers and collection of timed overnight urine samples, stored at -70°C, with control for urinary flow rate, age, sex and race in statistical models.
OBJECTIVE: To provide recommendations for design and analysis of studies using urine specimens to evaluate renal function or mercury excretion in children. METHODS: An analysis of mercury, albumin, γ-glutamyl transpeptidase (γ-GT) and N-acetyl-β-D-glucosaminidase (NAG) concentrations was carried out. RESULTS:Mercury concentration and creatinine-corrected renal markers were higher in daytime compared with overnight samples. Excretion rates increased with urinary flow rate. γ-GT and NAG concentrations decreased with storage time at -20°C. Differences by age, sex and race were noted. CONCLUSIONS: We recommend use of these creatinine-corrected markers and collection of timed overnight urine samples, stored at -70°C, with control for urinary flow rate, age, sex and race in statistical models.
Authors: Camille A Jones; Mildred E Francis; Mark S Eberhardt; Blanche Chavers; Josef Coresh; Michael Engelgau; John W Kusek; Danita Byrd-Holt; K M Venkat Narayan; William H Herman; Camara P Jones; Marcel Salive; Lawrence Y Agodoa Journal: Am J Kidney Dis Date: 2002-03 Impact factor: 8.860