Literature DB >> 20861344

Broad detection of diverse H5 and H7 hemagglutinin genes of avian influenza viruses by real-time reverse transcription-PCR using primer and probe sets containing mixed bases.

Kenji Tsukamoto1, Daigo Noguchi, Koutaro Suzuki, Makiko Shishido, Takayoshi Ashizawa, Min-Chul Kim, Youn-Jeong Lee, Tatsuya Tada.   

Abstract

Real-time reverse transcription-PCR (RT-PCR) was developed for broad detection of diverse H5 and H7 genes in Eurasian and American lineages of avian influenza viruses by using primer and probe sets containing mixed bases. Optimal use of the mixed bases enabled us to minimize sequence mismatches and to broaden the gene detection spectrum without decreasing sensitivity.

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Year:  2010        PMID： 20861344      PMCID： PMC3020814          DOI： 10.1128/JCM.01264-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Rapid and highly sensitive pathotyping of avian influenza A H5N1 virus by using real-time reverse transcription-PCR.

Authors:  Bernd Hoffmann; Timm Harder; Elke Starick; Klaus Depner; Ortrud Werner; Martin Beer
Journal:  J Clin Microbiol       Date:  2006-12-20       Impact factor: 5.948

2.  Development and bench validation of real-time reverse transcription polymerase chain reaction protocols for rapid detection of the subtypes H6, H9, and H11 of avian influenza viruses in experimental samples.

Authors:  Amaresh Das; David L Suarez
Journal:  J Vet Diagn Invest       Date:  2007-11       Impact factor: 1.279

3.  Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.

Authors:  Kenji Tsukamoto; Takayoshi Ashizawa; Koji Nakanishi; Noriyuki Kaji; Kotaro Suzuki; Makiko Shishido; Masatoshi Okamatsu; Masaji Mase
Journal:  J Clin Microbiol       Date:  2009-04-29       Impact factor: 5.948

4.  Subtyping of avian influenza viruses H1 to H15 on the basis of hemagglutinin genes by PCR assay and molecular determination of pathogenic potential.

Authors:  Kenji Tsukamoto; Hisayoshi Ashizawa; Koji Nakanishi; Noriyuki Kaji; Kotaro Suzuki; Masatoshi Okamatsu; Shigeo Yamaguchi; Masaji Mase
Journal:  J Clin Microbiol       Date:  2008-07-02       Impact factor: 5.948

5.  Development and validation of a one-step real-time PCR assay for simultaneous detection of subtype H5, H7, and H9 avian influenza viruses.

Authors:  Isabella Monne; Silvia Ormelli; Annalisa Salviato; Cristian De Battisti; Francesca Bettini; Angela Salomoni; Alessandra Drago; Bianca Zecchin; Ilaria Capua; Giovanni Cattoli
Journal:  J Clin Microbiol       Date:  2008-03-26       Impact factor: 5.948

6.  Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test.

Authors:  L Mia Kim; Claudio L Afonso; David L Suarez
Journal:  J Vet Diagn Invest       Date:  2006-11       Impact factor: 1.279

7.  Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes.

Authors:  Erica Spackman; Dennis A Senne; T J Myers; Leslie L Bulaga; Lindsey P Garber; Michael L Perdue; Kenton Lohman; Luke T Daum; David L Suarez
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

8.  Analytical validation of a real-time reverse transcription polymerase chain reaction test for Pan-American lineage H7 subtype Avian influenza viruses.

Authors:  Erica Spackman; Hon S Ip; David L Suarez; Richard D Slemons; David E Stallknecht
Journal:  J Vet Diagn Invest       Date:  2008-09       Impact factor: 1.279
  8 in total
  5 in total

1.  Subpopulation Primers Essential for Exhaustive Detection of Diverse Hemagglutinin Genes of H5 Subtype Avian Influenza Viruses by Loop-Mediated Isothermal Amplification Method.

Authors:  Y Furuyama; Y Takahashi; K Noguchi; H Murakami; M Sakaguchi; Shin Hisamatsu; T Usui; T Yamaguchi; T Ito; K Tsukamoto
Journal:  J Clin Microbiol       Date:  2018-08-27       Impact factor: 5.948

2.  SYBR green-based real-time reverse transcription-PCR for typing and subtyping of all hemagglutinin and neuraminidase genes of avian influenza viruses and comparison to standard serological subtyping tests.

Authors:  Kenji Tsukamoto; Carlos Javier Panei; Panei Carlos Javier; Makiko Shishido; Daigo Noguchi; John Pearce; Hyun-Mi Kang; Ok Mi Jeong; Youn-Jeong Lee; Koji Nakanishi; Takayoshi Ashizawa
Journal:  J Clin Microbiol       Date:  2011-10-26       Impact factor: 5.948

3.  Emergence of avian influenza viruses with enhanced transcription activity by a single amino acid substitution in the nucleoprotein during replication in chicken brains.

Authors:  Tatsuya Tada; Koutaro Suzuki; Yu Sakurai; Masanori Kubo; Hironao Okada; Toshihiro Itoh; Kenji Tsukamoto
Journal:  J Virol       Date:  2011-07-27       Impact factor: 5.103

4.  Application of real-time reverse transcription polymerase chain reaction to the detection the matrix, H5 and H7 genes of avian influenza viruses in field samples from South Korea.

Authors:  Hye-Ryoung Kim; Jae-Ku Oem; You-Chan Bae; Min-Su Kang; Hee-Soo Lee; Yong-Kuk Kwon
Journal:  Virol J       Date:  2013-03-14       Impact factor: 4.099

Review 5.  Prevalence and control of H7 avian influenza viruses in birds and humans.

Authors:  E M Abdelwhab; J Veits; T C Mettenleiter
Journal:  Epidemiol Infect       Date:  2014-01-15       Impact factor: 4.434
  5 in total

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