OBJECTIVE: The aim of our study was to evaluate the lymphocyte subpopulations and the cytokines in the peripheral blood of patients with type-A Stanford acute aortic dissection (AAD group) and to determine whether inflammatory cells are present at the site of aortic dissection. METHODS: Thirty-five consecutive patients with type-A Stanford dissection were evaluated for haemochrome and lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD4+CD25+, CD16+CD56+, CD4+CD28-, CD8+CD28-) by flow cytometry. C-reactive protein (CRP), tumour necrosis factor (TNF)-α, interleukin (IL)-2, IL-4, IL-6, IL-8, IL-10, interferon-gamma (IFN-γ), and monocyte chemoattractant protein (MCP)-1 were measured by ELISA. In addition, immunohistochemical staining with cell type-specific antibodies was performed to study the inflammatory cells detected inside the aortic wall. RESULTS: In the AAD group, a significant increase in natural killer (NK) (P = 0.032), B cells (P = 0.022), and CD8+CD28- (P = 0.045) subpopulations was observed, whereas there was a significant decrease in total T lymphocytes (P = 0.004) and T helper fractions (P = 0.005). Moreover, a significant increase in CRP (P < 0.0001), IL-6 (P < 0.0001), IL-8 (P < 0.0001), IL-10 (P < 0.0001), TNF-α (P < 0.0001), and MCP-1 (P < 0.001) was observed; macrophages represented the main population detected inside the media. CONCLUSIONS: Our results strongly support the hypothesis of a pivotal role of innate immunity in type-A Stanford AAD.
OBJECTIVE: The aim of our study was to evaluate the lymphocyte subpopulations and the cytokines in the peripheral blood of patients with type-A Stanford acute aortic dissection (AAD group) and to determine whether inflammatory cells are present at the site of aortic dissection. METHODS: Thirty-five consecutive patients with type-A Stanford dissection were evaluated for haemochrome and lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD4+CD25+, CD16+CD56+, CD4+CD28-, CD8+CD28-) by flow cytometry. C-reactive protein (CRP), tumour necrosis factor (TNF)-α, interleukin (IL)-2, IL-4, IL-6, IL-8, IL-10, interferon-gamma (IFN-γ), and monocyte chemoattractant protein (MCP)-1 were measured by ELISA. In addition, immunohistochemical staining with cell type-specific antibodies was performed to study the inflammatory cells detected inside the aortic wall. RESULTS: In the AAD group, a significant increase in natural killer (NK) (P = 0.032), B cells (P = 0.022), and CD8+CD28- (P = 0.045) subpopulations was observed, whereas there was a significant decrease in total T lymphocytes (P = 0.004) and T helper fractions (P = 0.005). Moreover, a significant increase in CRP (P < 0.0001), IL-6 (P < 0.0001), IL-8 (P < 0.0001), IL-10 (P < 0.0001), TNF-α (P < 0.0001), and MCP-1 (P < 0.001) was observed; macrophages represented the main population detected inside the media. CONCLUSIONS: Our results strongly support the hypothesis of a pivotal role of innate immunity in type-A Stanford AAD.
Authors: F Del Porto; N Cifani; M Proietta; T Dezi; L Tritapepe; S Raffa; A Micaloni; M Taurino Journal: Clin Exp Med Date: 2019-07-13 Impact factor: 3.984
Authors: Ali Kemal Kalkan; Huseyin Altug Cakmak; Mehmet Emin Kalkan; Mehmet Altug Tuncer; Ebuzer Aydin; Mehmed Yanartas; Muhammet Hulusi Satilmisoglu; Hale Unal Aksu; Mehmet Erturk; Mehmet Gul; Ugur Arslantas; Mehmet Kaan Kirali Journal: Ann Noninvasive Electrocardiol Date: 2014-11-23 Impact factor: 1.468
Authors: Fanny Laroumanie; Arina Korneva; Matthew R Bersi; Matthew R Alexander; Liang Xiao; Xue Zhong; Justin P Van Beusecum; Yuhan Chen; Mohamed A Saleh; William G McMaster; Kyle A Gavulic; Bethany L Dale; Shilin Zhao; Yan Guo; Yu Shyr; Daniel S Perrien; Nancy J Cox; John A Curci; Jay D Humphrey; Meena S Madhur Journal: JCI Insight Date: 2018-10-18