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Literature DB >> 20854260

Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.

Clara Bermejo¹, Farzad Haerizadeh, Hitomi Takanaga, Diane Chermak, Wolf B Frommer.

Abstract

Precise and dynamic measurement of intracellular metabolite levels has been hampered by difficulties in differentiating between adsorbed and imported fractions and the subcellular distribution between cytosol, endomembrane compartments and mitochondria. In the present study, genetically encoded FRET (Förster resonance energy transfer)-based sensors were deployed for dynamic measurements of free cytosolic glucose and ATP with varying external supply and in glucose-transport mutants. Moreover, by using the FRET sensors in a microfluidic platform, we were able to monitor in vivo changes of intracellular free glucose in individual yeast cells. We demonstrate the suitability of the FRET sensors for gaining physiological insight by demonstrating that free intracellular glucose and ATP levels are reduced in a hxt5Δ hexose-transporter mutant compared with wild-type and other hxtΔ strains.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2010 PMID： 20854260 PMCID： PMC2992555 DOI： 10.1042/BJ20100946

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

34 in total

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Journal: Biol Cell Date: 2007-01 Impact factor: 4.458

8. GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor.

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Journal: Biochim Biophys Acta Date: 2007-12-14

9. Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization.

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Journal: Microbiology (Reading) Date: 2002-09 Impact factor: 2.777

10. Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators.

Authors: Hiromi Imamura; Kim P Huynh Nhat; Hiroko Togawa; Kenta Saito; Ryota Iino; Yasuyuki Kato-Yamada; Takeharu Nagai; Hiroyuki Noji
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36 in total

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Authors: Clara Bermejo; Farzad Haerizadeh; Hitomi Takanaga; Diane Chermak; Wolf B Frommer
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Journal: Neurophotonics Date: 2014-05-29 Impact factor: 3.593