Literature DB >> 20837018

Depletion of TGF-β from fetal bovine serum.

Takatoku Oida1, Howard L Weiner.   

Abstract

TGF-β is one of the key cytokines controlling immune responses. Measuring TGF-β from culture supernatants in vitro is an important index of immune function. However, fetal bovine serum (FBS) contains a high level of latent TGF-β that often hampers measuring T cell-derived TGF-β in culture using FBS-supplemented medium. In this report, we generated anti-latency associated peptide (LAP) monoclonal antibodies which cross-react with bovine LAP, and developed a protocol to deplete TGF-β from FBS. This provides the ability to reliably quantify TGF-β in vitro without relying on serum-free media which do not support growth of murine T cells.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20837018      PMCID： PMC2989462          DOI： 10.1016/j.jim.2010.09.008

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  3 in total

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