Literature DB >> 2483947

Sandwich enzyme-linked immunosorbent assays (SELISAs) quantitate and distinguish two forms of transforming growth factor-beta (TGF-beta 1 and TGF-beta 2) in complex biological fluids.

D Danielpour1, K Y Kim, L L Dart, S Watanabe, A B Roberts, M B Sporn.   

Abstract

We have developed sandwich enzyme-linked immunosorbent assays (SELISAs) for TGF-beta 1 and TGF-beta 2 using both turkey and rabbit neutralizing polyclonal antibodies against native TGF-beta s. Each assay is based on the binding of two different antibodies to distinct epitopes of a single TGF-beta molecule. With these assays, TGF-beta types 1 and 2 can each be specifically quantitated in complex biological fluids, with detection limits of 2-5 pg. TGF-beta 3 and TGF-beta 5 either do not cross-react or cross-react very poorly in these assays. TGF-beta 1.2 heterodimer, although 50-80% neutralized by either TGF-beta 1 or TGF-beta 2 antibodies, shows only a 1.5 and 3.7% cross-reactivity in the TGF-beta 1 and TGF-beta 2 SELISAs, respectively. The SELISAs reported here represent the most specific, rapid, and precise assays for TGF-beta 1 and TGF-beta 2 reported thus far.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2483947     DOI： 10.3109/08977198909069082

Source DB:  PubMed          Journal:  Growth Factors        ISSN： 0897-7194            Impact factor:   2.511


  26 in total

Review 1.  The extracellular regulation of growth factor action.

Authors:  R Flaumenhaft; D B Rifkin
Journal:  Mol Biol Cell       Date:  1992-10       Impact factor: 4.138

2.  The recombinant proregion of transforming growth factor beta1 (latency-associated peptide) inhibits active transforming growth factor beta1 in transgenic mice.

Authors:  E P Böttinger; V M Factor; M L Tsang; J A Weatherbee; J B Kopp; S W Qian; L M Wakefield; A B Roberts; S S Thorgeirsson; M B Sporn
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

3.  Elevated transforming growth factor β1 in plasma of primary open-angle glaucoma patients.

Authors:  John Kuchtey; Jessica Kunkel; L Goodwin Burgess; Megan B Parks; Milam A Brantley; Rachel W Kuchtey
Journal:  Invest Ophthalmol Vis Sci       Date:  2014-07-24       Impact factor: 4.799

4.  Keratinocyte growth factor decreases pulmonary edema, transforming growth factor-beta and platelet-derived growth factor-BB expression, and alveolar type II cell loss in bleomycin-induced lung injury.

Authors:  E S Yi; M Salgado; S Williams; S J Kim; E Masliah; S Yin; T R Ulich
Journal:  Inflammation       Date:  1998-06       Impact factor: 4.092

5.  Binding and biologic characterization of recombinant human serum albumin-eTGFBR2 fusion protein expressed in CHO cells.

Authors:  Aini Wan; Yana Miao; Lin Peng; Yanfei Cai; Yun Chen; Yang He; Jianfeng Yang; Jian Jin; Huazhong Li
Journal:  Bioengineered       Date:  2017-02-17       Impact factor: 3.269

6.  Regulation of alveolar macrophage transforming growth factor-beta secretion by corticosteroids in bleomycin-induced pulmonary inflammation in the rat.

Authors:  N Khalil; C Whitman; L Zuo; D Danielpour; A Greenberg
Journal:  J Clin Invest       Date:  1993-10       Impact factor: 14.808

7.  Localization of transforming growth factor-beta 1 in mitochondria of murine heart and liver.

Authors:  U I Heine; J K Burmester; K C Flanders; D Danielpour; E F Munoz; A B Roberts; M B Sporn
Journal:  Cell Regul       Date:  1991-06

8.  Suppressor T cells generated by oral tolerization to myelin basic protein suppress both in vitro and in vivo immune responses by the release of transforming growth factor beta after antigen-specific triggering.

Authors:  A Miller; O Lider; A B Roberts; M B Sporn; H L Weiner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

9.  Cyclic AMP induces transforming growth factor beta 2 gene expression and growth arrest in the human androgen-independent prostate carcinoma cell line PC-3.

Authors:  Y J Bang; S J Kim; D Danielpour; M A O'Reilly; K Y Kim; C E Myers; J B Trepel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-15       Impact factor: 11.205

10.  Human cytomegalovirus infection induces transcription and secretion of transforming growth factor beta 1.

Authors:  S Michelson; J Alcami; S J Kim; D Danielpour; F Bachelerie; L Picard; C Bessia; C Paya; J L Virelizier
Journal:  J Virol       Date:  1994-09       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.