PURPOSE: we analyzed the sex chromosome-encoding ZFX-ZFY genes and tested molecular sexing using the amplification patterns of intron 9 of ZFX-ZFY in the horse. METHODS AND RESULTS: the amplification of the ZFX-ZFY produced two distinct patterns, reflecting sexual dimorphism based on a length difference between the X and Y chromosomes. The amplification products from foals showed two distinct bands: one was common to all foals and mares, indicating that this band was amplified from ZFX, while the other was specific to some foals, indicating that it was from ZFY. The result based on the PCR assay was identical to the results of amplification of the Y chromosome-specific SRY gene and those of investigations of the phenotypic gender in three different horse populations. CONCLUSION: we suggest that this PCR strategy for determining sexes by comparing the amplification patterns of ZFX-ZFY genes is a convenient and precise method for discriminating sexes in horses.
PURPOSE: we analyzed the sex chromosome-encoding ZFX-ZFY genes and tested molecular sexing using the amplification patterns of intron 9 of ZFX-ZFY in the horse. METHODS AND RESULTS: the amplification of the ZFX-ZFY produced two distinct patterns, reflecting sexual dimorphism based on a length difference between the X and Y chromosomes. The amplification products from foals showed two distinct bands: one was common to all foals and mares, indicating that this band was amplified from ZFX, while the other was specific to some foals, indicating that it was from ZFY. The result based on the PCR assay was identical to the results of amplification of the Y chromosome-specific SRY gene and those of investigations of the phenotypic gender in three different horse populations. CONCLUSION: we suggest that this PCR strategy for determining sexes by comparing the amplification patterns of ZFX-ZFY genes is a convenient and precise method for discriminating sexes in horses.
Authors: A H Sinclair; P Berta; M S Palmer; J R Hawkins; B L Griffiths; M J Smith; J W Foster; A M Frischauf; R Lovell-Badge; P N Goodfellow Journal: Nature Date: 1990-07-19 Impact factor: 49.962
Authors: Kimberly Glass; John Quackenbush; Edwin K Silverman; Bartolome Celli; Stephen I Rennard; Guo-Cheng Yuan; Dawn L DeMeo Journal: BMC Syst Biol Date: 2014-11-28
Authors: Susana A Teixeira; Adriana M G Ibelli; Maurício E Cantão; Haniel C de Oliveira; Mônica C Ledur; Jane de O Peixoto; Daniele B D Marques; Karine A Costa; Luiz L Coutinho; Simone E F Guimarães Journal: Genes (Basel) Date: 2019-12-05 Impact factor: 4.096