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Literature DB >> 208059

RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions.

Abstract

Rates of formation of RNA:DNA hybrids have been measured as a function of temperature and compared to DNA:RNA duplex denaturation temperatures in 4 M sodium perchlorate, 4 M NaClO4-6 M urea, and 3 M rubidium trichloracetate solvents. The usual bell shaped curves of reaction rate versus temperature were observed. The optimal temperatures for the RNA:DNA association reaction are 5 degrees to 12 degrees greater than the Tm's for DNA:DNA denaturation in these solvents, just as in formamide. R-loops of phi80d3ilv DNA with E. coli rRNA can be formed at high efficiency in these solvents.

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Year: 1978 PMID： 208059 PMCID： PMC342109 DOI： 10.1093/nar/5.5.1627

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

1. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

Authors: J Casey; N Davidson
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

2. Interaction of nucleosides and related compounds with nucleic acids as indicated by the change of helix-coil transition temperature.

Authors: P O TS'O; G K HELMKAMP; C SANDER
Journal: Proc Natl Acad Sci U S A Date: 1962-04-15 Impact factor: 11.205

3. Underwound loops in self-renatured DNA can be diagnostic of inverted duplications and translocated sequences.

Authors: T R Broker; L Soll; L T Chow
Journal: J Mol Biol Date: 1977-07-15 Impact factor: 5.469

4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

Review 5. Genetics of bacterial ribosomes.

Authors: M Nomura; E A Morgan
Journal: Annu Rev Genet Date: 1977 Impact factor: 16.830

6. Positions of sea urchin (Strongylocentrotus purpuratus) histone genes relative to restriction endonuclease sites on the chimeric plasmids pSp2 and pSp17.

Authors: D S Holmes; R H Cohn; L H Kedes; N Davidson
Journal: Biochemistry Date: 1977-04-05 Impact factor: 3.162

RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions.

1. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.

2. Interaction of nucleosides and related compounds with nucleic acids as indicated by the change of helix-coil transition temperature.

3. Underwound loops in self-renatured DNA can be diagnostic of inverted duplications and translocated sequences.

4. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Review 5. Genetics of bacterial ribosomes.

6. Positions of sea urchin (Strongylocentrotus purpuratus) histone genes relative to restriction endonuclease sites on the chimeric plasmids pSp2 and pSp17.

7. Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant.

8. Hybridization of RNA to double-stranded DNA: formation of R-loops.

9. Nucleic acid reassociation in formamide.

10. Dependence of the melting temperature of DNA on salt concentration.

1. The use of oligodeoxynucleotide probes in chaotrope-based hybridization solutions.

2. Bacterial RNA:DNA hybrids are activators of the NLRP3 inflammasome.

3. Advances in the Detection of Toxic Algae Using Electrochemical Biosensors.

4. Comparison of rRNA depletion methods for efficient bacterial mRNA sequencing.

5. The Fanconi Anemia Pathway Maintains Genome Stability by Coordinating Replication and Transcription.