Literature DB >> 20805030

Condensed E. coli cultures for highly efficient production of proteins containing unnatural amino acids.

Jia Liu1, Carlos A Castañeda, Bryan J Wilkins, David Fushman, T Ashton Cropp.   

Abstract

Current biosynthetic methods for producing proteins containing site-specifically incorporated unnatural amino acids are inefficient because the majority of the amino acid goes unused. Here we present a universal approach to improve the efficiency of such processes using condensed Escherichia coli cultures. Copyright (c) 2010 Elsevier Ltd. All rights reserved.

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Year:  2010        PMID： 20805030      PMCID： PMC2941765          DOI： 10.1016/j.bmcl.2010.08.049

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  16 in total

1.  Engineering and characterization of a superfolder green fluorescent protein.

Authors:  Jean-Denis Pédelacq; Stéphanie Cabantous; Timothy Tran; Thomas C Terwilliger; Geoffrey S Waldo
Journal:  Nat Biotechnol       Date:  2005-12-20       Impact factor: 54.908

2.  Single protein production (SPP) system in Escherichia coli.

Authors:  Motoo Suzuki; Lili Mao; Masayori Inouye
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

3.  Peptide mass fingerprinting using isotopically encoded photo-crosslinking amino acids.

Authors:  Bryan J Wilkins; Kelly A Daggett; T Ashton Cropp
Journal:  Mol Biosyst       Date:  2008-07-17

4.  Bacterial bioreactors for high yield production of recombinant protein.

Authors:  Motoo Suzuki; Rohini Roy; Haiyan Zheng; Nancy Woychik; Masayori Inouye
Journal:  J Biol Chem       Date:  2006-10-04       Impact factor: 5.157

5.  The incorporation of a photoisomerizable amino acid into proteins in E. coli.

Authors:  Mohua Bose; Dan Groff; Jianming Xie; Eric Brustad; Peter G Schultz
Journal:  J Am Chem Soc       Date:  2006-01-18       Impact factor: 15.419

6.  Adding amino acids with novel reactivity to the genetic code of Saccharomyces cerevisiae.

Authors:  Alexander Deiters; T Ashton Cropp; Mridul Mukherji; Jason W Chin; J Christopher Anderson; Peter G Schultz
Journal:  J Am Chem Soc       Date:  2003-10-01       Impact factor: 15.419

7.  Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases.

Authors:  Takahito Mukai; Takatsugu Kobayashi; Nobumasa Hino; Tatsuo Yanagisawa; Kensaku Sakamoto; Shigeyuki Yokoyama
Journal:  Biochem Biophys Res Commun       Date:  2008-05-08       Impact factor: 3.575

8.  Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli.

Authors:  Jason W Chin; Andrew B Martin; David S King; Lei Wang; Peter G Schultz
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-01       Impact factor: 11.205

9.  Genetically encoded photocontrol of protein localization in mammalian cells.

Authors:  Arnaud Gautier; Duy P Nguyen; Hrvoje Lusic; Wenlin An; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-03-31       Impact factor: 15.419

10.  Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion.

Authors:  Kaihang Wang; Heinz Neumann; Sew Y Peak-Chew; Jason W Chin
Journal:  Nat Biotechnol       Date:  2007-06-24       Impact factor: 54.908
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  9 in total

1.  A method for multi-codon scanning mutagenesis of proteins based on asymmetric transposons.

Authors:  Jia Liu; T Ashton Cropp
Journal:  Protein Eng Des Sel       Date:  2011-12-18       Impact factor: 1.650

2.  Nonenzymatic assembly of natural polyubiquitin chains of any linkage composition and isotopic labeling scheme.

Authors:  Carlos Castañeda; Jia Liu; Apurva Chaturvedi; Urszula Nowicka; T Ashton Cropp; David Fushman
Journal:  J Am Chem Soc       Date:  2011-10-19       Impact factor: 15.419

Review 3.  Unraveling the complexity of ubiquitin signaling.

Authors:  Eric R Strieter; David A Korasick
Journal:  ACS Chem Biol       Date:  2012-01-11       Impact factor: 5.100

4.  Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups.

Authors:  Carlos A Castañeda; Jia Liu; Tanuja R Kashyap; Rajesh K Singh; David Fushman; T Ashton Cropp
Journal:  Chem Commun (Camb)       Date:  2011-01-06       Impact factor: 6.222

5.  Structure-based non-canonical amino acid design to covalently crosslink an antibody-antigen complex.

Authors:  Jianqing Xu; Drew Tack; Randall A Hughes; Andrew D Ellington; Jeffrey J Gray
Journal:  J Struct Biol       Date:  2013-05-13       Impact factor: 2.867

6.  Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication.

Authors:  Tyler L Dangerfield; Kenneth A Johnson
Journal:  J Biol Chem       Date:  2020-10-05       Impact factor: 5.157

7.  Selective immunocapture and light-controlled traceless release of transiently caged proteins.

Authors:  Rasa Rakauskaitė; Giedrė Urbanavičiūtė; Martynas Simanavičius; Aurelija Žvirblienė; Saulius Klimašauskas
Journal:  STAR Protoc       Date:  2021-04-14

8.  Site-specific bioconjugation of a murine dihydrofolate reductase enzyme by copper(I)-catalyzed azide-alkyne cycloaddition with retained activity.

Authors:  Sung In Lim; Yukina Mizuta; Akinori Takasu; Yong Hwan Kim; Inchan Kwon
Journal:  PLoS One       Date:  2014-06-02       Impact factor: 3.240

9.  The "gate keeper" role of Trp222 determines the enantiopreference of diketoreductase toward 2-chloro-1-phenylethanone.

Authors:  Hairong Ma; Xin Yang; Zhuo Lu; Nan Liu; Yijun Chen
Journal:  PLoS One       Date:  2014-07-29       Impact factor: 3.240
  9 in total

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