| Literature DB >> 20735989 |
Cecy Ying-Chuck Kou1, Samantha Lai-Yee Lau, Ka-Wing Au, Pui-Yin Leung, Stephen Siu-Chung Chim, Kwok-Pui Fung, Mary Miu-Yee Waye, Stephen Kwok-Wing Tsui.
Abstract
The relationship between DNA methylation, histone modifications and terminal differentiation in cardiomyocytes was investigated in this study. The upregulation of methylation-related proteins, including DNA methyltransferase (DNMT) 1, methyl-CpG binding domain proteins 1, 2 and 3, and the increase in global methylation during rat neonatal heart development were observed. Moreover, an increase in DNA synthesis and a delay in differentiation were found in 5-azacytidine (5-azaC)-treated cardiomyocytes. Increase in acetylation of H3-K9, H4-K5, H4-K8 and methylation of H3-K4 suggested a more accessible chromatin structure in 5-azaC-treated cells. Furthermore, methyl-CpG-binding protein 2 was found to be upregulated in differentiated cardiomyocytes. Overexpression of this protein resulted in an increase of global methylation levels. Therefore, we suggest that a hypermethylated genome and a more compact chromatin structure are formed during terminal differentiation of cardiomyocytes.Entities:
Mesh：
Substances：
Year: 2010 PMID： 20735989 DOI： 10.1016/j.bbrc.2010.08.064
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575