Literature DB >> 20733045

Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.

Claire M Martel1, Josie E Parker, Andrew G S Warrilow, Nicola J Rolley, Steven L Kelly, Diane E Kelly.   

Abstract

Aspergillus fumigatus sterol 14α-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, recombinant YUG37-pcyp51A and YUG37-pcyp51B yeasts were able to synthesize ergosterol and grow; a control strain harboring reverse-oriented cyp51A could not. YUG37-pcyp51A and YUG37-pcyp51B constructs showed identical sensitivity to itraconazole, posaconazole, clotrimazole, and voriconazole. Conversely, YUG37-pcyp51A withstood 16-fold-higher concentrations of fluconazole than YUG37-pcyp51B (8 and 0.5 μg ml⁻¹, respectively).

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Year:  2010        PMID： 20733045      PMCID： PMC2976139          DOI： 10.1128/AAC.00349-10

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  36 in total

1.  Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

Authors:  E Mellado; G Garcia-Effron; M J Buitrago; L Alcazar-Fuoli; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2005-06       Impact factor: 5.191

2.  In vitro evolution of itraconazole resistance in Aspergillus fumigatus involves multiple mechanisms of resistance.

Authors:  Márcia Eliana da Silva Ferreira; José Luiz Capellaro; Everaldo dos Reis Marques; Iran Malavazi; David Perlin; Steven Park; James B Anderson; Arnaldo L Colombo; Beth A Arthington-Skaggs; Maria Helena S Goldman; Gustavo H Goldman
Journal:  Antimicrob Agents Chemother       Date:  2004-11       Impact factor: 5.191

3.  Metabolic control analysis and engineering of the yeast sterol biosynthetic pathway.

Authors:  Philip Groeneveld; Nicola Rolley; Douglas B Kell; Steven L Kelly; Diane E Kelly
Journal:  Mol Biol Rep       Date:  2002       Impact factor: 2.316

4.  The G464S amino acid substitution in Candida albicans sterol 14alpha-demethylase causes fluconazole resistance in the clinic through reduced affinity.

Authors:  S L Kelly; D C Lamb; J Loeffler; H Einsele; D E Kelly
Journal:  Biochem Biophys Res Commun       Date:  1999-08-19       Impact factor: 3.575

5.  Y132H substitution in Candida albicans sterol 14alpha-demethylase confers fluconazole resistance by preventing binding to haem.

Authors:  S L Kelly; D C Lamb; D E Kelly
Journal:  FEMS Microbiol Lett       Date:  1999-11-15       Impact factor: 2.742

6.  Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species.

Authors:  E Mellado; T M Diaz-Guerra; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

7.  Amphotericin B- and fluconazole-resistant Candida spp., Aspergillus fumigatus, and other newly emerging pathogenic fungi are susceptible to basic antifungal peptides.

Authors:  E J Helmerhorst; I M Reijnders; W van't Hof; I Simoons-Smit; E C Veerman; A V Amerongen
Journal:  Antimicrob Agents Chemother       Date:  1999-03       Impact factor: 5.191

8.  A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus.

Authors:  T M Diaz-Guerra; E Mellado; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2003-03       Impact factor: 5.191

9.  Mutations in Aspergillus fumigatus resulting in reduced susceptibility to posaconazole appear to be restricted to a single amino acid in the cytochrome P450 14alpha-demethylase.

Authors:  Paul A Mann; Raulo M Parmegiani; Shui-Qing Wei; Cara A Mendrick; Xin Li; David Loebenberg; Beth DiDomenico; Roberta S Hare; Scott S Walker; Paul M McNicholas
Journal:  Antimicrob Agents Chemother       Date:  2003-02       Impact factor: 5.191

10.  Essential gene identification and drug target prioritization in Aspergillus fumigatus.

Authors:  Wenqi Hu; Susan Sillaots; Sebastien Lemieux; John Davison; Sarah Kauffman; Anouk Breton; Annie Linteau; Chunlin Xin; Joel Bowman; Jeff Becker; Bo Jiang; Terry Roemer
Journal:  PLoS Pathog       Date:  2007-03       Impact factor: 6.823
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  17 in total

Review 1.  Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?

Authors:  Steven L Kelly; Diane E Kelly
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2013-01-06       Impact factor: 6.237

2.  Facultative sterol uptake in an ergosterol-deficient clinical isolate of Candida glabrata harboring a missense mutation in ERG11 and exhibiting cross-resistance to azoles and amphotericin B.

Authors:  Claire M Hull; Josie E Parker; Oliver Bader; Michael Weig; Uwe Gross; Andrew G S Warrilow; Diane E Kelly; Steven L Kelly
Journal:  Antimicrob Agents Chemother       Date:  2012-05-21       Impact factor: 5.191

3.  Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.

Authors:  K H M Nazmul Hussain Nazir; Hirofumi Ichinose; Hiroyuki Wariishi
Journal:  Appl Environ Microbiol       Date:  2011-03-04       Impact factor: 4.792

Review 4.  Resistance to antifungals that target CYP51.

Authors:  Josie E Parker; Andrew G S Warrilow; Claire L Price; Jonathan G L Mullins; Diane E Kelly; Steven L Kelly
Journal:  J Chem Biol       Date:  2014-08-27

5.  Gene Amplification of CYP51B: a New Mechanism of Resistance to Azole Compounds in Trichophyton indotineae.

Authors:  Tsuyoshi Yamada; Takashi Yaguchi; Mari Maeda; Mohamed Mahdi Alshahni; Karine Salamin; Emmanuella Guenova; Marc Feuermann; Michel Monod
Journal:  Antimicrob Agents Chemother       Date:  2022-05-12       Impact factor: 5.938

Review 6.  Molecular Tools for the Detection and Deduction of Azole Antifungal Drug Resistance Phenotypes in Aspergillus Species.

Authors:  Anna Dudakova; Birgit Spiess; Marut Tangwattanachuleeporn; Christoph Sasse; Dieter Buchheidt; Michael Weig; Uwe Groß; Oliver Bader
Journal:  Clin Microbiol Rev       Date:  2017-10       Impact factor: 26.132

7.  In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus.

Authors:  Andrew G S Warrilow; Josie E Parker; Claire L Price; W David Nes; Steven L Kelly; Diane E Kelly
Journal:  Antimicrob Agents Chemother       Date:  2015-10-12       Impact factor: 5.191

8.  High-frequency triazole resistance found In nonculturable Aspergillus fumigatus from lungs of patients with chronic fungal disease.

Authors:  David W Denning; Steven Park; Cornelia Lass-Florl; Marcin G Fraczek; Marie Kirwan; Robin Gore; Jaclyn Smith; Ahmed Bueid; Caroline B Moore; Paul Bowyer; David S Perlin
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

9.  Analysis of the cyp51 genes contribution to azole resistance in Aspergillus section Nigri with the CRISPR-Cas9 technique.

Authors:  Alba Pérez-Cantero; Adela Martin-Vicente; Josep Guarro; Jarrod R Fortwendel; Javier Capilla
Journal:  Antimicrob Agents Chemother       Date:  2021-03-08       Impact factor: 5.191

10.  Ergosterol biosynthesis in Aspergillus fumigatus: its relevance as an antifungal target and role in antifungal drug resistance.

Authors:  Laura Alcazar-Fuoli; Emilia Mellado
Journal:  Front Microbiol       Date:  2013-01-10       Impact factor: 5.640
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