Literature DB >> 15917566

Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility.

E Mellado1, G Garcia-Effron, M J Buitrago, L Alcazar-Fuoli, M Cuenca-Estrella, J L Rodriguez-Tudela.   

Abstract

The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.

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Year:  2005        PMID： 15917566      PMCID： PMC1140498          DOI： 10.1128/AAC.49.6.2536-2538.2005

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  15 in total

1.  Inoculum standardization for antifungal susceptibility testing of filamentous fungi pathogenic for humans.

Authors:  E Petrikkou; J L Rodríguez-Tudela; M Cuenca-Estrella; A Gómez; A Molleja; E Mellado
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

2.  Influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of Candida spp.

Authors:  M Cuenca-Estrella; T M Díaz-Guerra; E Mellado; J L Rodríguez-Tudela
Journal:  J Clin Microbiol       Date:  2001-02       Impact factor: 5.948

3.  Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph.

Authors:  D Cullen; S A Leong; L J Wilson; D J Henner
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Quantitation of Candida albicans ergosterol content improves the correlation between in vitro antifungal susceptibility test results and in vivo outcome after fluconazole treatment in a murine model of invasive candidiasis.

Authors:  B A Arthington-Skaggs; D W Warnock; C J Morrison
Journal:  Antimicrob Agents Chemother       Date:  2000-08       Impact factor: 5.191

5.  Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species.

Authors:  E Mellado; T M Diaz-Guerra; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

6.  Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis.

Authors:  J M Smith; C M Tang; S Van Noorden; D W Holden
Journal:  Infect Immun       Date:  1994-12       Impact factor: 3.441

7.  In vitro and in vivo effects of 14alpha-demethylase (ERG11) depletion in Candida glabrata.

Authors:  H Nakayama; N Nakayama; M Arisawa; Y Aoki
Journal:  Antimicrob Agents Chemother       Date:  2001-11       Impact factor: 5.191

8.  Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5'-monophosphate decarboxylase.

Authors:  G Weidner; C d'Enfert; A Koch; P C Mol; A A Brakhage
Journal:  Curr Genet       Date:  1998-05       Impact factor: 3.886

9.  Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility.

Authors:  A Geber; C A Hitchcock; J E Swartz; F S Pullen; K E Marsden; K J Kwon-Chung; J E Bennett
Journal:  Antimicrob Agents Chemother       Date:  1995-12       Impact factor: 5.191

10.  Substitutions at methionine 220 in the 14alpha-sterol demethylase (Cyp51A) of Aspergillus fumigatus are responsible for resistance in vitro to azole antifungal drugs.

Authors:  E Mellado; G Garcia-Effron; L Alcazar-Fuoli; M Cuenca-Estrella; J L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2004-07       Impact factor: 5.191
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  56 in total

1.  Identification of novel genes conferring altered azole susceptibility in Aspergillus fumigatus.

Authors:  Paul Bowyer; Juan Mosquera; Michael Anderson; Mike Birch; Michael Bromley; David W Denning
Journal:  FEMS Microbiol Lett       Date:  2012-05-21       Impact factor: 2.742

2.  SREBP-dependent triazole susceptibility in Aspergillus fumigatus is mediated through direct transcriptional regulation of erg11A (cyp51A).

Authors:  Sara J Blosser; Robert A Cramer
Journal:  Antimicrob Agents Chemother       Date:  2011-10-17       Impact factor: 5.191

3.  Time of incubation for antifungal susceptibility testing of Aspergillus fumigatus: can MIC values be obtained at 24 hours?

Authors:  Juan Luis Rodriguez-Tudela; Laura Alcazar-Fuoli; Ana Alastruey-Izquierdo; Araceli Monzon; Emilia Mellado; Manuel Cuenca-Estrella
Journal:  Antimicrob Agents Chemother       Date:  2007-10-15       Impact factor: 5.191

4.  An alternative host model of a mixed fungal infection by azole susceptible and resistant Aspergillus spp strains.

Authors:  L Alcazar-Fuoli; Mj Buitrago; A Gomez-Lopez; E Mellado
Journal:  Virulence       Date:  2015       Impact factor: 5.882

5.  Role of Aspergillus lentulus 14-α sterol demethylase (Cyp51A) in azole drug susceptibility.

Authors:  Emilia Mellado; Laura Alcazar-Fuoli; Manuel Cuenca-Estrella; Juan L Rodriguez-Tudela
Journal:  Antimicrob Agents Chemother       Date:  2011-09-26       Impact factor: 5.191

6.  Aspergillus fumigatus Cyp51A and Cyp51B Proteins Are Compensatory in Function and Localize Differentially in Response to Antifungals and Cell Wall Inhibitors.

Authors:  Mark T Roundtree; Praveen R Juvvadi; E Keats Shwab; D Christopher Cole; William J Steinbach
Journal:  Antimicrob Agents Chemother       Date:  2020-09-21       Impact factor: 5.191

7.  In vitro survey of triazole cross-resistance among more than 700 clinical isolates of Aspergillus species.

Authors:  M A Pfaller; S A Messer; L Boyken; C Rice; S Tendolkar; R J Hollis; D J Diekema
Journal:  J Clin Microbiol       Date:  2008-06-18       Impact factor: 5.948

8.  Novel yeast-based strategy unveils antagonist binding regions on the nuclear xenobiotic receptor PXR.

Authors:  Hao Li; Matthew R Redinbo; Madhukumar Venkatesh; Sean Ekins; Anik Chaudhry; Nicolin Bloch; Abdissa Negassa; Paromita Mukherjee; Ganjam Kalpana; Sridhar Mani
Journal:  J Biol Chem       Date:  2013-03-22       Impact factor: 5.157

9.  Development of azole resistance in Aspergillus fumigatus during azole therapy associated with change in virulence.

Authors:  Maiken Cavling Arendrup; Eleftheria Mavridou; Klaus Leth Mortensen; Eveline Snelders; Niels Frimodt-Møller; Humara Khan; Willem J G Melchers; Paul E Verweij
Journal:  PLoS One       Date:  2010-04-09       Impact factor: 3.240

10.  Wild-type MIC distribution and epidemiological cutoff values for Aspergillus fumigatus and three triazoles as determined by the Clinical and Laboratory Standards Institute broth microdilution methods.

Authors:  M A Pfaller; D J Diekema; M A Ghannoum; J H Rex; B D Alexander; D Andes; S D Brown; V Chaturvedi; A Espinel-Ingroff; C L Fowler; E M Johnson; C C Knapp; M R Motyl; L Ostrosky-Zeichner; D J Sheehan; T J Walsh
Journal:  J Clin Microbiol       Date:  2009-08-19       Impact factor: 5.948
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