| Literature DB >> 20728564 |
Hui Shi1, Qigui Wang, Yuxiang Wang, Li Leng, Qingqiu Zhang, Zhouchun Shang, Hui Li.
Abstract
Fatty acid-binding proteins (FABPs) may facilitate the diffusion of fatty acids within the cytoplasm. Adipocyte FABP (A-FABP) null mice exhibit altered rates of lipolysis, and reprogrammed expression of genes related to lipid metabolism. In the current study, chicken adipocytes with short hairpin RNA-mediated knockdown and cDNA overexpression of A-FABP were used to investigate the role of chicken A-FABP in lipid metabolism of adipocytes. The results showed that there was no significant change of lipid metabolism in the A-FABP interference adipocytes. However, in the A-FABP overexpression adipocytes, the lipolysis was increased significantly at 36 h (p<0.05), and lipid accumulation was increased significantly at 48, 60 and 72 h (p<0.05). The expression of PPAR γ and perilipin genes were upregulated, while the expression of E-FABP gene was downregulated after transfection with pcDNA3.1/A-FABP (p<0.05). The present study suggested that A-FABP gene might affect the lipid metabolism through the PPAR γ pathway, and the function between A-FABP and E-FABP gene was compensatory in the chickens.Entities:
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Year: 2010 PMID： 20728564 DOI： 10.1016/j.cbpb.2010.08.005
Source DB: PubMed Journal: Comp Biochem Physiol B Biochem Mol Biol ISSN： 1096-4959 Impact factor: 2.231