Literature DB >> 20717776

Proteomic analysis of saliva: 2D gel electrophoresis, LC-MS/MS, and Western blotting.

Shen Hu1, Jiang Jiang, David T Wong.   

Abstract

Saliva harbors a wide spectrum of proteins that may reflect the health/disease status in the human body. Profiling of the proteins in saliva from a disease population can potentially yield valuable clinical parameters to be used for diagnosis and prognosis of the disease. Advances in proteomic technologies have enabled comprehensive profiling of protein expression in cells, tissue, and body fluids. When applied to readily accessible saliva samples from disease patients for biomarker study, such a global approach allows attaining the most discriminatory protein biomarkers that can best predict the disease status. In this chapter, we describe the protocols for proteomic analysis of saliva using 2D gel electrophoresis, Western blotting, and LC-MS/MS.

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Year:  2010        PMID： 20717776      PMCID： PMC3209956          DOI： 10.1007/978-1-60761-820-1_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

1.  Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches.

Authors:  Yuan Yao; Eric A Berg; Catherine E Costello; Robert F Troxler; Frank G Oppenheim
Journal:  J Biol Chem       Date:  2002-11-19       Impact factor: 5.157

2.  Characterization of the human salivary proteome by capillary isoelectric focusing/nanoreversed-phase liquid chromatography coupled with ESI-tandem MS.

Authors:  Tong Guo; Paul A Rudnick; Weijie Wang; Cheng S Lee; Don L Devoe; Brian M Balgley
Journal:  J Proteome Res       Date:  2006-06       Impact factor: 4.466

3.  The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions.

Authors:  Paul Denny; Fred K Hagen; Markus Hardt; Lujian Liao; Weihong Yan; Martha Arellanno; Sara Bassilian; Gurrinder S Bedi; Pinmannee Boontheung; Daniel Cociorva; Claire M Delahunty; Trish Denny; Jason Dunsmore; Kym F Faull; Joyce Gilligan; Mireya Gonzalez-Begne; Frédéric Halgand; Steven C Hall; Xuemei Han; Bradley Henson; Johannes Hewel; Shen Hu; Sherry Jeffrey; Jiang Jiang; Joseph A Loo; Rachel R Ogorzalek Loo; Daniel Malamud; James E Melvin; Olga Miroshnychenko; Mahvash Navazesh; Richard Niles; Sung Kyu Park; Akraporn Prakobphol; Prasanna Ramachandran; Megan Richert; Sarah Robinson; Melissa Sondej; Puneet Souda; Mark A Sullivan; Jona Takashima; Shawn Than; Jianghua Wang; Julian P Whitelegge; H Ewa Witkowska; Lawrence Wolinsky; Yongming Xie; Tao Xu; Weixia Yu; Jimmy Ytterberg; David T Wong; John R Yates; Susan J Fisher
Journal:  J Proteome Res       Date:  2008-03-25       Impact factor: 4.466

4.  Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.

Authors:  Prasanna Ramachandran; Pinmannee Boontheung; Yongming Xie; Melissa Sondej; David T Wong; Joseph A Loo
Journal:  J Proteome Res       Date:  2006-06       Impact factor: 4.466

5.  Proteomic study of salivary peptides and proteins in patients with Sjögren's syndrome before and after pilocarpine treatment.

Authors:  G Peluso; M De Santis; R Inzitari; C Fanali; T Cabras; I Messana; M Castagnola; G F Ferraccioli
Journal:  Arthritis Rheum       Date:  2007-07

6.  Salivary proteomic and genomic biomarkers for primary Sjögren's syndrome.

Authors:  Shen Hu; Jianghua Wang; Jiska Meijer; Sonya Ieong; Yongming Xie; Tianwei Yu; Hui Zhou; Sharon Henry; Arjan Vissink; Justin Pijpe; Cees Kallenberg; David Elashoff; Joseph A Loo; David T Wong
Journal:  Arthritis Rheum       Date:  2007-11

7.  Systematic comparison of the human saliva and plasma proteomes.

Authors:  Weihong Yan; Rolf Apweiler; Brian M Balgley; Pinmanee Boontheung; Jonathan L Bundy; Benjamin J Cargile; Steve Cole; Xueping Fang; Mireya Gonzalez-Begne; Timothy J Griffin; Fred Hagen; Shen Hu; Lawrence E Wolinsky; Cheng S Lee; Daniel Malamud; James E Melvin; Rajasree Menon; Michael Mueller; Renli Qiao; Nelson L Rhodus; Joel R Sevinsky; David States; James L Stephenson; Shawn Than; John R Yates; Weixia Yu; Hongwei Xie; Yongming Xie; Gilbert S Omenn; Joseph A Loo; David T Wong
Journal:  Proteomics Clin Appl       Date:  2009-01-01       Impact factor: 3.494

8.  Proteomics analysis of cells in whole saliva from oral cancer patients via value-added three-dimensional peptide fractionation and tandem mass spectrometry.

Authors:  Hongwei Xie; Getiria Onsongo; Jonathan Popko; Ebbing P de Jong; Jing Cao; John V Carlis; Robert J Griffin; Nelson L Rhodus; Timothy J Griffin
Journal:  Mol Cell Proteomics       Date:  2007-11-28       Impact factor: 5.911

9.  Tyrosine polysulfation of human salivary histatin 1. A post-translational modification specific of the submandibular gland.

Authors:  Tiziana Cabras; Chiara Fanali; Joana A Monteiro; Francisco Amado; Rosanna Inzitari; Claudia Desiderio; Emanuele Scarano; Bruno Giardina; Massimo Castagnola; Irene Messana
Journal:  J Proteome Res       Date:  2007-05-16       Impact factor: 4.466

10.  Trafficking and postsecretory events responsible for the formation of secreted human salivary peptides: a proteomics approach.

Authors:  Irene Messana; Tiziana Cabras; Elisabetta Pisano; Maria Teresa Sanna; Alessandra Olianas; Barbara Manconi; Mariagiuseppina Pellegrini; Gaetano Paludetti; Emanuele Scarano; Antonella Fiorita; Stefania Agostino; Alessia M Contucci; Lea Calò; Pasqualina M Picciotti; Armando Manni; Anders Bennick; Alberto Vitali; Chiara Fanali; Rosanna Inzitari; Massimo Castagnola
Journal:  Mol Cell Proteomics       Date:  2008-01-09       Impact factor: 5.911
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  9 in total

Review 1.  Defining salivary biomarkers using mass spectrometry-based proteomics: a systematic review.

Authors:  Sandra K Al-Tarawneh; Michael B Border; Christopher F Dibble; Sompop Bencharit
Journal:  OMICS       Date:  2011-05-13

Review 2.  The scientific exploration of saliva in the post-proteomic era: from database back to basic function.

Authors:  Stefan Ruhl
Journal:  Expert Rev Proteomics       Date:  2012       Impact factor: 3.940

Review 3.  Salivary extracellular noncoding RNA: emerging biomarkers for molecular diagnostics.

Authors:  David T W Wong
Journal:  Clin Ther       Date:  2015-03-18       Impact factor: 3.393

Review 4.  Protein-based biomarkers in cerebrospinal fluid and blood for Alzheimer's disease.

Authors:  Yongyao Fu; Deming Zhao; Lifeng Yang
Journal:  J Mol Neurosci       Date:  2014-06-25       Impact factor: 3.444

Review 5.  Non-coding RNAs in saliva: emerging biomarkers for molecular diagnostics.

Authors:  Blanca Majem; Marina Rigau; Jaume Reventós; David T Wong
Journal:  Int J Mol Sci       Date:  2015-04-17       Impact factor: 5.923

6.  More Accurate Oral Cancer Screening with Fewer Salivary Biomarkers.

Authors:  James Michael Menke; Md Shahidul Ahsan; Suan Phaik Khoo
Journal:  Biomark Cancer       Date:  2017-10-17

7.  Determination of soluble angiotensin-converting enzyme 2 in saliva samples and its association with nicotine.

Authors:  Samuel Bru; Adrián González-Marrón; Cristina Lidón-Moyano; Reyes Carballar; Joan Marc Martínez-Láinez; Hipólito Pérez-Martín; Marcela Fu; Raúl Pérez-Ortuño; Montse Ballbè; Jose A Pascual; Esteve Fernández; Josep Clotet; Jose M Martínez-Sánchez
Journal:  Environ Res       Date:  2022-10-03       Impact factor: 8.431

8.  Variation of Human Salivary O-Glycome.

Authors:  Radoslaw P Kozak; Paulina A Urbanowicz; Chamindie Punyadeera; Karli R Reiding; Bas C Jansen; Louise Royle; Daniel I Spencer; Daryl L Fernandes; Manfred Wuhrer
Journal:  PLoS One       Date:  2016-09-09       Impact factor: 3.240

Review 9.  Salivary Biomarkers in Systemic Sclerosis Disease.

Authors:  Zeineb Zian; Joaira Bakkach; Amina Barakat; Naima Ghailani Nourouti; Mohcine Bennani Mechita
Journal:  Biomed Res Int       Date:  2018-03-12       Impact factor: 3.411
  9 in total

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