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Literature DB >> 20711534

A genetically encoded photocaged Nepsilon-methyl-L-lysine.

Yane-Shih Wang¹, Bo Wu, Zhiyong Wang, Ying Huang, Wei Wan, William K Russell, Pei-Jing Pai, Yin N Moe, David H Russell, Wenshe R Liu.

Abstract

A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

Entities: Chemical Species

Mesh：

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Year: 2010 PMID： 20711534 DOI： 10.1039/c002155e

Source DB: PubMed Journal: Mol Biosyst ISSN： 1742-2051

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Cited

37 in total

Review 1. Designer proteins: applications of genetic code expansion in cell biology.

Authors: Lloyd Davis; Jason W Chin
Journal: Nat Rev Mol Cell Biol Date: 2012-02-15 Impact factor: 94.444

2. A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

Authors: Yane-Shih Wang; Xinqiang Fang; Ashley L Wallace; Bo Wu; Wenshe R Liu
Journal: J Am Chem Soc Date: 2012-02-06 Impact factor: 15.419

3. A Versatile Approach for Site-Specific Lysine Acylation in Proteins.

Authors: Zhipeng A Wang; Yadagiri Kurra; Xin Wang; Yu Zeng; Yan-Jiun Lee; Vangmayee Sharma; Hening Lin; Susie Y Dai; Wenshe R Liu
Journal: Angew Chem Int Ed Engl Date: 2017-01-02 Impact factor: 15.336

4. Proteins with Site-Specific Lysine Methylation.

Authors: Zhipeng A Wang; Wenshe R Liu
Journal: Chemistry Date: 2017-08-01 Impact factor: 5.236

Review 5. Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

Authors: Wei Wan; Jeffery M Tharp; Wenshe R Liu
Journal: Biochim Biophys Acta Date: 2014-03-12

6. Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids.

Authors: Bo Wu; Zhiyong Wang; Ying Huang; Wenshe R Liu
Journal: Chembiochem Date: 2012-05-24 Impact factor: 3.164

A genetically encoded photocaged Nepsilon-methyl-L-lysine.

Review 1. Designer proteins: applications of genetic code expansion in cell biology.

2. A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

3. A Versatile Approach for Site-Specific Lysine Acylation in Proteins.

4. Proteins with Site-Specific Lysine Methylation.

Review 5. Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool.

6. Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two genetically incorporated noncanonical amino acids.

Review 7. Chemical biology approaches for studying posttranslational modifications.

8. A Chemical Biology Approach to Reveal Sirt6-targeted Histone H3 Sites in Nucleosomes.

9. Towards reassigning the rare AGG codon in Escherichia coli.

Review 10. Histone-binding domains: strategies for discovery and characterization.