BACKGROUND: Prostate specific antigen (PSA) measurement is used for the diagnosis of prostate cancer (PCa) but the test lacks specificity due to the number of false positive readings. The glycosylation of PSA is altered in PCa but studies in this area have been limited to few clinical samples and/or require advanced laboratory facilities. An assay to assess PSA glycosylation was established using equipment available in most routine biomedical testing laboratories. METHODS: Serum samples from patients with PCa or benign prostatic hyperplasia (BPH) were used. PSA (range 4-10 ng/ml) was affinity purified, separated and probed with the lectin Ulex europaeus (UEA-1; specific for α1,2 linked fucose). An enzyme-linked immunosorbent lectin assay (ELLA) with colorimetric detection was devised and PSA fucosylation assessed in a further independent set of 26 samples. RESULTS: Free PSA (fPSA) from PCa patients showed a significant increase in fucosylation compared with fPSA from patients with BPH. The ELLA was 92% specific and 69% sensitive for PCa over BPH. In comparison, fPSA measurement was 70% specific and 56% sensitive (threshold set to 25% tPSA) for PCa over BPH. CONCLUSIONS: Changes in glycosylation of PSA were identified using 50 μl of serum with PSA in the range of 4-10 ng/ml, this represents a more specific and sensitive test for PCa based on fucosylation changes of fPSA.
BACKGROUND:Prostate specific antigen (PSA) measurement is used for the diagnosis of prostate cancer (PCa) but the test lacks specificity due to the number of false positive readings. The glycosylation of PSA is altered in PCa but studies in this area have been limited to few clinical samples and/or require advanced laboratory facilities. An assay to assess PSA glycosylation was established using equipment available in most routine biomedical testing laboratories. METHODS: Serum samples from patients with PCa or benign prostatic hyperplasia (BPH) were used. PSA (range 4-10 ng/ml) was affinity purified, separated and probed with the lectin Ulex europaeus (UEA-1; specific for α1,2 linked fucose). An enzyme-linked immunosorbent lectin assay (ELLA) with colorimetric detection was devised and PSA fucosylation assessed in a further independent set of 26 samples. RESULTS: Free PSA (fPSA) from PCa patients showed a significant increase in fucosylation compared with fPSA from patients with BPH. The ELLA was 92% specific and 69% sensitive for PCa over BPH. In comparison, fPSA measurement was 70% specific and 56% sensitive (threshold set to 25% tPSA) for PCa over BPH. CONCLUSIONS: Changes in glycosylation of PSA were identified using 50 μl of serum with PSA in the range of 4-10 ng/ml, this represents a more specific and sensitive test for PCa based on fucosylation changes of fPSA.
Authors: Lúcia Patrícia Bezerra Gomes da Silva; Sinara Mônica Vitalino de Almeida; Luiza Rayanna Amorim de Lima; Carmelita de Lima Bezerra Cavalcanti; Mariana Montenegro de Melo Lira; Maria da Paz Carvalho da Silva; Eduardo Isidoro Carneiro Beltrão; Luiz Bezerra de Carvalho Júnior Journal: Int J Clin Exp Pathol Date: 2014-06-15
Authors: Jan Tkac; Veronika Gajdosova; Stefania Hroncekova; Tomas Bertok; Michal Hires; Eduard Jane; Lenka Lorencova; Peter Kasak Journal: Interface Focus Date: 2019-02-15 Impact factor: 3.906
Authors: Benjamin F Mann; John A Goetz; Michael G House; C Max Schmidt; Milos V Novotny Journal: Mol Cell Proteomics Date: 2012-03-05 Impact factor: 5.911
Authors: Bryan S Reatini; Elliot Ensink; Brian Liau; Jessica Y Sinha; Thomas W Powers; Katie Partyka; Marshall Bern; Randall E Brand; Pauline M Rudd; Doron Kletter; Richard Drake; Brian B Haab Journal: Anal Chem Date: 2016-11-15 Impact factor: 6.986