Literature DB >> 20700818

Heteronuclear relaxation in time-dependent spin systems: (15)N-T1 (rho) dispersion during adiabatic fast passage.

R Konrat1, M Tollinger.   

Abstract

A novel NMR experiment comprising adiabatic fast passage techniques for the measurement of heteronuclear self-relaxation rates in fully 15N-enriched proteins is described. Heteronuclear self-relaxation is monitored by performing adiabatic fast passage (AFP) experiments at variable adiabaticity (e.g., variation of RF spin-lock field intensity). The experiment encompasses gradient- selection and sensitivity-enhancement. It is shown that transverse relaxation rates derived with this method are in good agreement with the ones measured by the classical Carr-Purcell-Meiboom-Gill (CPMG) sequences. An application of this method to the study of the carboxyl-terminal LIM domain of quail cysteine and glycine-rich protein qCRP2(LIM2) is presented.

Entities:  

Year:  1999        PMID： 20700818     DOI： 10.1023/A:1008324721356

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  14 in total

1.  Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motions.

Authors:  S Zinn-Justin; P Berthault; M Guenneugues; H Desvaux
Journal:  J Biomol NMR       Date:  1997-12       Impact factor: 2.835

2.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

Review 3.  Investigation of protein motions via relaxation measurements.

Authors:  J W Peng; G Wagner
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

4.  Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain.

Authors:  M Akke; J Liu; J Cavanagh; H P Erickson; A G Palmer
Journal:  Nat Struct Biol       Date:  1998-01

5.  Backbone dynamics, amide hydrogen exchange, and resonance assignments of the DNA methylphosphotriester repair domain of Escherichia coli Ada using NMR.

Authors:  J Habazettl; L C Myers; F Yuan; G L Verdine; G Wagner
Journal:  Biochemistry       Date:  1996-07-23       Impact factor: 3.162

6.  Protein dynamics studied by rotating frame 15N spin relaxation times.

Authors:  T Szyperski; P Luginbühl; G Otting; P Güntert; K Wüthrich
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

7.  Backbone dynamics of proteins as studied by 15N inverse detected heteronuclear NMR spectroscopy: application to staphylococcal nuclease.

Authors:  L E Kay; D A Torchia; A Bax
Journal:  Biochemistry       Date:  1989-11-14       Impact factor: 3.162

8.  Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.

Authors:  N A Farrow; R Muhandiram; A U Singer; S M Pascal; C M Kay; G Gish; S E Shoelson; T Pawson; J D Forman-Kay; L E Kay
Journal:  Biochemistry       Date:  1994-05-17       Impact factor: 3.162

9.  Backbone dynamics of (1-71)bacterioopsin studied by two-dimensional 1H-15N NMR spectroscopy.

Authors:  K V Pervushin; A S Arseniev
Journal:  Eur J Biochem       Date:  1994-02-01

10.  The cysteine-rich protein family of highly related LIM domain proteins.

Authors:  R Weiskirchen; J D Pino; T Macalma; K Bister; M C Beckerle
Journal:  J Biol Chem       Date:  1995-12-01       Impact factor: 5.157
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  6 in total

1.  Heteronuclear Adiabatic Relaxation Dispersion (HARD) for quantitative analysis of conformational dynamics in proteins.

Authors:  Nathaniel J Traaseth; Fa-An Chao; Larry R Masterson; Silvia Mangia; Michael Garwood; Shalom Michaeli; Burckhard Seelig; Gianluigi Veglia
Journal:  J Magn Reson       Date:  2012-04-06       Impact factor: 2.229

2.  Mathematical treatment of adiabatic fast passage pulses for the computation of nuclear spin relaxation rates in proteins with conformational exchange.

Authors:  Renate Auer; Martin Tollinger; Ilya Kuprov; Robert Konrat; Karin Kloiber
Journal:  J Biomol NMR       Date:  2011-09-27       Impact factor: 2.835

Review 3.  Characterizing micro-to-millisecond chemical exchange in nucleic acids using off-resonance R1ρ relaxation dispersion.

Authors:  Atul Rangadurai; Eric S Szymaski; Isaac J Kimsey; Honglue Shi; Hashim M Al-Hashimi
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2019-05-11       Impact factor: 9.795

4.  Phosphorus-31 transverse relaxation rate measurements by NMR spectroscopy: insight into conformational exchange along the nucleic acid backbone.

Authors:  Laurent J Catoire
Journal:  J Biomol NMR       Date:  2004-02       Impact factor: 2.835

Review 5.  NMR contributions to structural dynamics studies of intrinsically disordered proteins.

Authors:  Robert Konrat
Journal:  J Magn Reson       Date:  2014-04       Impact factor: 2.229

6.  Cross-correlated relaxation measurements under adiabatic sweeps: determination of local order in proteins.

Authors:  Pavel Kadeřávek; Sarina Grutsch; Nicola Salvi; Martin Tollinger; Lukáš Žídek; Geoffrey Bodenhausen; Fabien Ferrage
Journal:  J Biomol NMR       Date:  2015-10-28       Impact factor: 2.835
  6 in total

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