AIMS: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. METHODS AND RESULTS: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1/stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2(+) , eae(+) , ehxA(+) virulence pattern. CONCLUSIONS: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. SIGNIFICANCE AND IMPACT OF THE STUDY: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.
AIMS: To feno-genotypically characterize the Shiga toxin-producing Escherichia coli (STEC) population in Argentinean dairy cows. METHODS AND RESULTS: From 540 STEC positive samples, 170 isolates were analyzed by multiplex PCR and serotyping. Of these, 11% carried stx1, 52% stx2 and 37% stx1/stx2. The ehxA, saa and eae were detected in 77%, 66% and 3%, respectively. Thirty-five per cent of strains harboured the profile stx1, stx2, saa, ehxA and 29% stx2, saa, ehxA. One hundred and fifty-six strains were associated with 29 different O serogroups, and 19 H antigens were distributed among 157 strains. STEC O113:H21, O130:H11 and O178:H19 were the most frequently found serotypes. The STEC O157:H7 were detected in low rate and corresponded to the stx2(+) , eae(+) , ehxA(+) virulence pattern. CONCLUSIONS: We detected a diversity of STEC strains in dairy cattle from Argentina, most of them carrying genes linked to human disease. SIGNIFICANCE AND IMPACT OF THE STUDY: The non-O157 STEC serotypes described in this study are associated worldwide with disease in humans and represent a risk for the public health. For this, any microbiological control in dairy farms should be targeted not only to the search of O157:H7 serotype.
Authors: Rosana Polifroni; Analía I Etcheverría; Marcelo E Sanz; Rosana E Cepeda; Alejandra Krüger; Paula M A Lucchesi; Daniel Fernández; Alberto E Parma; Nora L Padola Journal: Curr Microbiol Date: 2012-06-16 Impact factor: 2.188
Authors: María E Cáceres; Analía I Etcheverría; Daniel Fernández; Edgardo M Rodríguez; Nora L Padola Journal: Front Cell Infect Microbiol Date: 2017-04-28 Impact factor: 5.293
Authors: Daniel Fernández; Alejandra Krüger; Rosana Polifroni; Ana V Bustamante; A Mariel Sanso; Analía I Etcheverría; Paula M A Lucchesi; Alberto E Parma; Nora L Padola Journal: Front Cell Infect Microbiol Date: 2013-03-08 Impact factor: 5.293
Authors: Ana V Bustamante; Andrea M Sanso; Alberto E Parma; Paula M A Lucchesi Journal: Front Cell Infect Microbiol Date: 2012-08-22 Impact factor: 5.293