Literature DB >> 20680573

Improved protocol for high-quality co-extraction of DNA and RNA from rumen digesta.

M Popova1, C Martin, D P Morgavi.   

Abstract

We report an improved method for total nucleic acids extraction from rumen content samples. The method employs bead beating, and phenol-chloroform extraction followed by saline-alcohol precipitation. Total nucleic acids and RNA yield and purity were assessed by spectrophotometric measurements; RNA integrity was estimated using Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer. The method provided total nucleic acids and RNA extracts of good quantity and quality. The extraction is not time consuming and it is valuable for ecological studies of rumen microbial community structure and gene expression.

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Year:  2010        PMID： 20680573     DOI： 10.1007/s12223-010-0060-3

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  10 in total

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Authors:  Seungha Kang; Stuart E Denman; Mark Morrison; Zhongtang Yu; Chris S McSweeney
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5.  Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil.

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Review 6.  The regulation of ribosomal RNA synthesis and bacterial cell growth.

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7.  Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms.

Authors:  Y Q Guo; J-X Liu; Y Lu; W Y Zhu; S E Denman; C S McSweeney
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8.  Development of a RT-qPCR method for the quantification of Fibrobacter succinogenes S85 glycoside hydrolase transcripts in the rumen content of gnotobiotic and conventional sheep.

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Journal:  J Microbiol Methods       Date:  2009-01-31       Impact factor: 2.363

Review 9.  Direct extraction of DNA from soils for studies in microbial ecology.

Authors:  Mark A Schneegurt; Sophia Y Dore; Charles F Kulpa
Journal:  Curr Issues Mol Biol       Date:  2003-01       Impact factor: 2.081

10.  The RIN: an RNA integrity number for assigning integrity values to RNA measurements.

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  10 in total
  11 in total

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Journal:  Appl Environ Microbiol       Date:  2019-02-06       Impact factor: 4.792

2.  Evaluation of DNA extraction methods of rumen microbial populations.

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5.  Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources.

Authors:  Letícia M Campos; Vinícius C Souza; Yury T Granja-Salcedo; Juliana D Messana; Jacquelyn M Prestegaard-Wilson; Maria Júlia G Ganga; Ana Veronica L Dias; Vladimir E Costa; Telma T Berchielli
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6.  Methanogens and methanogenesis in the rumens and ceca of lambs fed two different high-grain-content diets.

Authors:  M Popova; D P Morgavi; C Martin
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7.  The use of live yeast to increase intake and performance of cattle receiving low-quality tropical forages.

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8.  Changes in Rumen Microbial Community Composition during Adaption to an In Vitro System and the Impact of Different Forages.

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9.  The Structural and Functional Capacity of Ruminal and Cecal Microbiota in Growing Cattle Was Unaffected by Dietary Supplementation of Linseed Oil and Nitrate.

Authors:  Milka Popova; Emily McGovern; Matthew S McCabe; Cécile Martin; Michel Doreau; Marie Arbre; Sarah J Meale; Diego P Morgavi; Sinéad M Waters
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10.  Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta.

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