Literature DB >> 20680568

PCR-DGGE analysis of bacterial population attached to the bovine rumen wall.

F Lukás1, J Simůnek, J Mrázek, J Kopecný.   

Abstract

We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken from each animal. The DGGE profile of the bacterial population attached to the rumen wall represented a high inter-animal variation.

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Year:  2010        PMID: 20680568     DOI: 10.1007/s12223-010-0055-0

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN: 0015-5632            Impact factor:   2.099


  13 in total

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Authors:  S Sadet; C Martin; B Meunier; D P Morgavi
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Journal:  Appl Environ Microbiol       Date:  1978-01       Impact factor: 4.792

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