| Literature DB >> 20603019 |
Xiaoying Bai1, Jonghwan Kim, Zhongan Yang, Michael J Jurynec, Thomas E Akie, Joseph Lee, Jocelyn LeBlanc, Anna Sessa, Hong Jiang, Anthony DiBiase, Yi Zhou, David J Grunwald, Shuo Lin, Alan B Cantor, Stuart H Orkin, Leonard I Zon.
Abstract
Recent genome-wide studies have demonstrated that pausing of RNA polymerase II (Pol II) occurred on many vertebrate genes. By genetic studies in the zebrafish tif1gamma mutant moonshine we found that loss of function of Pol II-associated factors PAF or DSIF rescued erythroid gene transcription in tif1gamma-deficient animals. Biochemical analysis established physical interactions among TIF1gamma, the blood-specific SCL transcription complex, and the positive elongation factors p-TEFb and FACT. Chromatin immunoprecipitation assays in human CD34(+) cells supported a TIF1gamma-dependent recruitment of positive elongation factors to erythroid genes to promote transcription elongation by counteracting Pol II pausing. Our study establishes a mechanism for regulating tissue cell fate and differentiation through transcription elongation. Copyright 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20603019 PMCID： PMC3072682 DOI： 10.1016/j.cell.2010.05.028
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582