Literature DB >> 20600511

Bluetongue virus RNA detection by RT-qPCR in blood samples of sheep vaccinated with a commercially available inactivated BTV-8 vaccine.

Adolf Steinrigl1, Sandra Revilla-Fernández, Michaela Eichinger, Josef Koefer, Petra Winter.   

Abstract

In 2008, a country-wide bluetongue virus 8 (BTV-8) vaccination campaign has been initiated in Austria, using a single commercial inactivated BTV-8 vaccine. Based on preliminary data, we hypothesised that vaccine-derived BTV RNA is transiently detectable by reverse transcription quantitative real-time PCR (RT-qPCR) in the blood of vaccinated animals. Thus BTV-8 vaccine was administered to five BTV-naïve adult sheep and blood samples were taken at various time-points post-vaccination. BTV RNA was detectable by several RT-qPCR methods in all five animals, mainly within the first 9 days post-vaccination. These results show that RT-qPCR based testing for BTV-infection may be influenced by vaccination with certain inactivated BTV-8 vaccines. Copyright 2010 Elsevier Ltd. All rights reserved.

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Year:  2010        PMID： 20600511     DOI： 10.1016/j.vaccine.2010.06.034

Source DB:  PubMed          Journal:  Vaccine        ISSN： 0264-410X            Impact factor:   3.641


  7 in total

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Journal:  J Vet Diagn Invest       Date:  2020-09-30       Impact factor: 1.279

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Authors:  Kathy Toohey-Kurth; Monica M Reising; Rebecca L Tallmadge; Laura B Goodman; Jianfa Bai; Steven R Bolin; Janice C Pedersen; Mangkey A Bounpheng; Roman M Pogranichniy; Jane Christopher-Hennings; Mary Lea Killian; Donna M Mulrooney; Roger Maes; Shri Singh; Beate M Crossley
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3.  Rapid generation of replication-deficient monovalent and multivalent vaccines for bluetongue virus: protection against virulent virus challenge in cattle and sheep.

Authors:  Cristina C P Celma; Mark Boyce; Piet A van Rijn; Michael Eschbaumer; Kerstin Wernike; Bernd Hoffmann; Martin Beer; Andy Haegeman; Kris De Clercq; Polly Roy
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6.  The Bluetongue Disabled Infectious Single Animal (DISA) Vaccine Platform Based on Deletion NS3/NS3a Protein Is Safe and Protective in Cattle and Enables DIVA.

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Journal:  Viruses       Date:  2021-05-07       Impact factor: 5.048

7.  A Qualitative Risk Assessment for Bluetongue Disease and African Horse Sickness: The Risk of Entry and Exposure at a UK Zoo.

Authors:  Elisabeth Nelson; William Thurston; Paul Pearce-Kelly; Hannah Jenkins; Mary Cameron; Simon Carpenter; Amanda Guthrie; Marion England
Journal:  Viruses       Date:  2022-02-28       Impact factor: 5.818
  7 in total

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