Literature DB >> 20592150

Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis.

Kristyna Hrncirova1, Martina Lengerova, Iva Kocmanova, Zdenek Racil, Pavlina Volfova, Dita Palousova, Mojmir Moulis, Barbora Weinbergerova, Jana Winterova, Martina Toskova, Sarka Pospisilova, Jiri Mayer.   

Abstract

We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

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Year:  2010        PMID： 20592150      PMCID： PMC2937726          DOI： 10.1128/JCM.01109-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

Review 1.  Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies.

Authors:  U Lehmann; H Kreipe
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

2.  Determination of RhD zygosity: comparison of a double amplification refractory mutation system approach and a multiplex real-time quantitative PCR approach.

Authors:  R W Chiu; M F Murphy; C Fidler; B C Zee; J S Wainscoat; Y M Lo
Journal:  Clin Chem       Date:  2001-04       Impact factor: 8.327

Review 3.  High-resolution DNA melting analysis for simple and efficient molecular diagnostics.

Authors:  Gudrun H Reed; Jana O Kent; Carl T Wittwer
Journal:  Pharmacogenomics       Date:  2007-06       Impact factor: 2.533

Review 4.  High resolution melting applications for clinical laboratory medicine.

Authors:  Maria Erali; Karl V Voelkerding; Carl T Wittwer
Journal:  Exp Mol Pathol       Date:  2008-04-13       Impact factor: 3.362

5.  Real-time PCR method for detection of zygomycetes.

Authors:  D Jane Hata; Seanne P Buckwalter; Bobbi S Pritt; Glenn D Roberts; Nancy L Wengenack
Journal:  J Clin Microbiol       Date:  2008-05-14       Impact factor: 5.948

6.  Caspofungin inhibits Rhizopus oryzae 1,3-beta-D-glucan synthase, lowers burden in brain measured by quantitative PCR, and improves survival at a low but not a high dose during murine disseminated zygomycosis.

Authors:  Ashraf S Ibrahim; Joel C Bowman; Valentina Avanessian; Keturah Brown; Brad Spellberg; John E Edwards; Cameron M Douglas
Journal:  Antimicrob Agents Chemother       Date:  2005-02       Impact factor: 5.191

Review 7.  Zygomycetes in human disease.

Authors:  J A Ribes; C L Vanover-Sams; D J Baker
Journal:  Clin Microbiol Rev       Date:  2000-04       Impact factor: 26.132

8.  DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients.

Authors:  Birgit Spiess; Wolfgang Seifarth; Margit Hummel; Oliver Frank; Alice Fabarius; Chun Zheng; Handan Mörz; Rüdiger Hehlmann; Dieter Buchheidt
Journal:  J Clin Microbiol       Date:  2007-08-22       Impact factor: 5.948

9.  Detection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis.

Authors:  Miki Kasai; Susan M Harrington; Andrea Francesconi; Vidmantas Petraitis; Ruta Petraitiene; Mara G Beveridge; Tena Knudsen; Jeffery Milanovich; Margaret P Cotton; Johanna Hughes; Robert L Schaufele; Tin Sein; John Bacher; Patrick R Murray; Dimitrios P Kontoyiannis; Thomas J Walsh
Journal:  J Clin Microbiol       Date:  2008-09-24       Impact factor: 5.948

10.  Comparison of histopathological analysis, culture, and polymerase chain reaction assays to detect invasive mold infections from biopsy specimens.

Authors:  Volker Rickerts; Sabine Mousset; Evelyn Lambrecht; Kathrin Tintelnot; Rainer Schwerdtfeger; Elisabeth Presterl; Volkmar Jacobi; Gudrun Just-Nübling; Ralf Bialek
Journal:  Clin Infect Dis       Date:  2007-03-05       Impact factor: 9.079
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  14 in total

1.  Biopsy procedures for molecular tissue diagnosis of invasive fungal infections.

Authors:  Cornelia Mrazek; Cornelia Lass-Flörl
Journal:  Curr Infect Dis Rep       Date:  2011-12       Impact factor: 3.725

2.  Rapid detection and identification of mucormycetes in bronchoalveolar lavage samples from immunocompromised patients with pulmonary infiltrates by use of high-resolution melt analysis.

Authors:  Martina Lengerova; Zdenek Racil; Kristyna Hrncirova; Iva Kocmanova; Pavlina Volfova; Dita Ricna; Petr Bejdak; Mojmir Moulis; Zdenek Pavlovsky; Barbora Weinbergerova; Martina Toskova; Jiri Mayer
Journal:  J Clin Microbiol       Date:  2014-05-21       Impact factor: 5.948

3.  Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.

Authors:  María Alonso; Pilar Escribano; Jesus Guinea; Sandra Recio; Ainhoa Simon; Teresa Peláez; Emilio Bouza; Darío García de Viedma
Journal:  J Clin Microbiol       Date:  2012-07-25       Impact factor: 5.948

Review 4.  Microbiological applications of high-resolution melting analysis.

Authors:  Steven Y C Tong; Philip M Giffard
Journal:  J Clin Microbiol       Date:  2012-08-08       Impact factor: 5.948

5.  High-resolution melting analysis assay for identification of Fonsecaea species.

Authors:  Minglan Shi; Xiqing Li; Jiao Feng; Shulin Jia; Xing Xiao; Chunmei Chen; Cindy Fransisca; Liyan Xi; Junmin Zhang
Journal:  J Clin Lab Anal       Date:  2017-05-22       Impact factor: 2.352

Review 6.  [PCR-based diagnosis of mucormycosis in tissue samples].

Authors:  R Bialek; U E Zelck
Journal:  Pathologe       Date:  2013-11       Impact factor: 1.011

7.  Rapid identification of medically important Candida isolates using high resolution melting analysis.

Authors:  Eva Nemcova; Michaela Cernochova; Filip Ruzicka; Barbora Malisova; Tomas Freiberger; Petr Nemec
Journal:  PLoS One       Date:  2015-02-17       Impact factor: 3.240

8.  Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis?

Authors:  Hagen Frickmann; Ulrike Loderstaedt; Paul Racz; Klara Tenner-Racz; Petra Eggert; Alexandra Haeupler; Ralf Bialek; Ralf Matthias Hagen
Journal:  Biomed Res Int       Date:  2015-04-19       Impact factor: 3.411

Review 9.  Cutaneous mucormycosis.

Authors:  Ana Daniela Castrejón-Pérez; Esperanza C Welsh; Ivett Miranda; Jorge Ocampo-Candiani; Oliverio Welsh
Journal:  An Bras Dermatol       Date:  2017 May-Jun       Impact factor: 1.896

10.  Dynamic time warping assessment of high-resolution melt curves provides a robust metric for fungal identification.

Authors:  Sha Lu; Gordana Mirchevska; Sayali S Phatak; Dongmei Li; Janos Luka; Richard A Calderone; William A Fonzi
Journal:  PLoS One       Date:  2017-03-06       Impact factor: 3.240
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