Literature DB >> 20589527

Comparison of EMSA and SPR for the characterization of RNA-RNase II complexes.

Rute G Matos1, Ana Barbas, Cecília M Arraiano.   

Abstract

RNases are enzymes that process and degrade RNA molecules. As such, the study of the interactions between these enzymes and RNA molecules is essential in order to better understand their mechanism of action. In this report, our aim was to use E. coli RNase II as a model to compare two different techniques for the characterization and interpretation of the stability of RNA-protein complexes: Surface Plasmon Resonance and Electrophoretic Mobility Shift Assay.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20589527     DOI： 10.1007/s10930-010-9265-1

Source DB:  PubMed          Journal:  Protein J        ISSN： 1572-3887            Impact factor:   2.371


  12 in total

Review 1.  Degradation of mRNA in bacteria: emergence of ubiquitous features.

Authors:  P Régnier; C M Arraiano
Journal:  Bioessays       Date:  2000-03       Impact factor: 4.345

Review 2.  The enzymes and control of eukaryotic mRNA turnover.

Authors:  Roy Parker; Haiwei Song
Journal:  Nat Struct Mol Biol       Date:  2004-02       Impact factor: 15.369

3.  Post-transcriptional control of gene expression: effectors of mRNA decay.

Authors:  Cecília Maria Arraiano; Lynne Elizabeth Maquat
Journal:  Mol Microbiol       Date:  2003-07       Impact factor: 3.501

4.  Unravelling the dynamics of RNA degradation by ribonuclease II and its RNA-bound complex.

Authors:  Carlos Frazão; Colin E McVey; Mónica Amblar; Ana Barbas; Clemens Vonrhein; Cecília M Arraiano; Maria A Carrondo
Journal:  Nature       Date:  2006-09-07       Impact factor: 49.962

5.  Determination of key residues for catalysis and RNA cleavage specificity: one mutation turns RNase II into a "SUPER-ENZYME".

Authors:  Ana Barbas; Rute G Matos; Mónica Amblar; Eduardo López-Viñas; Paulino Gomez-Puertas; Cecília M Arraiano
Journal:  J Biol Chem       Date:  2009-05-19       Impact factor: 5.157

Review 6.  The role of 3'-5' exoribonucleases in RNA degradation.

Authors:  José M Andrade; Vânia Pobre; Inês J Silva; Susana Domingues; Cecília M Arraiano
Journal:  Prog Mol Biol Transl Sci       Date:  2009       Impact factor: 3.622

7.  Characterizing ribonucleases in vitro examples of synergies between biochemical and structural analysis.

Authors:  Cecília Maria Arraiano; Ana Barbas; Mónica Amblar
Journal:  Methods Enzymol       Date:  2008       Impact factor: 1.600

8.  A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding.

Authors:  Mónica Amblar; Cecília M Arraiano
Journal:  FEBS J       Date:  2005-01       Impact factor: 5.542

Review 9.  Function, mechanism and regulation of bacterial ribonucleases.

Authors:  A W Nicholson
Journal:  FEMS Microbiol Rev       Date:  1999-06       Impact factor: 16.408

10.  New insights into the mechanism of RNA degradation by ribonuclease II: identification of the residue responsible for setting the RNase II end product.

Authors:  Ana Barbas; Rute G Matos; Mónica Amblar; Eduardo López-Viñas; Paulino Gomez-Puertas; Cecília M Arraiano
Journal:  J Biol Chem       Date:  2008-03-12       Impact factor: 5.157
View more
  7 in total

1.  Using competition assays to quantitatively model cooperative binding by transcription factors and other ligands.

Authors:  Jacob Peacock; James B Jaynes
Journal:  Biochim Biophys Acta Gen Subj       Date:  2017-08-01       Impact factor: 3.770

2.  Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa.

Authors:  MaryClare F Rollins; Jason T Schuman; Kirra Paulus; Habib S T Bukhari; Blake Wiedenheft
Journal:  Nucleic Acids Res       Date:  2015-02-27       Impact factor: 16.971

3.  Interaction of the histone mRNA hairpin with stem-loop binding protein (SLBP) and regulation of the SLBP-RNA complex by phosphorylation and proline isomerization.

Authors:  Minyou Zhang; TuKiet T Lam; Marco Tonelli; William F Marzluff; Roopa Thapar
Journal:  Biochemistry       Date:  2012-04-03       Impact factor: 3.162

4.  Characterization of a single-stranded DNA binding protein from Salmonella enterica serovar Typhimurium LT2.

Authors:  Yen-Hua Huang; Yen-Ling Lee; Cheng-Yang Huang
Journal:  Protein J       Date:  2011-02       Impact factor: 2.371

5.  Characterization of a single-stranded DNA-binding protein from Pseudomonas aeruginosa PAO1.

Authors:  Hau-Chern Jan; Yen-Ling Lee; Cheng-Yang Huang
Journal:  Protein J       Date:  2011-01       Impact factor: 2.371

6.  The Molecular Basis of TnrA Control by Glutamine Synthetase in Bacillus subtilis.

Authors:  Ksenia Hauf; Airat Kayumov; Felix Gloge; Karl Forchhammer
Journal:  J Biol Chem       Date:  2015-12-03       Impact factor: 5.157

7.  An improved method for surface immobilisation of RNA: application to small non-coding RNA-mRNA pairing.

Authors:  Helen A Vincent; Jack O Phillips; Charlotte A Henderson; Adam J Roberts; Carlanne M Stone; Charlotte E Mardle; Louise E Butt; Darren M Gowers; Andrew R Pickford; Anastasia J Callaghan
Journal:  PLoS One       Date:  2013-11-14       Impact factor: 3.240
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.