| Literature DB >> 20580469 |
Thaís Lourdes Santos Lacerda1, Patrícia Gomes Cardoso, Leonardo Augusto de Almeida, Ilana Lopes Baratella da Cunha Camargo, Daniela Almeida Freitas Afonso, Cyntia Cardoso Trant, Gilson Costa Macedo, Eleonora Campos, Silvio L Cravero, Suzana P Salcedo, Jean-Pierre Gorvel, Sérgio Costa Oliveira.
Abstract
Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence. Copyright 2010 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20580469 DOI： 10.1016/j.vaccine.2010.06.023
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641