| Literature DB >> 24994009 |
Junbo Zhang1, Shuanghong Yin, Fei Guo, Ren Meng, Chuangfu Chen, Hui Zhang, Zhiqiang Li, Qiang Fu, Huijun Shi, Shengwei Hu, Wei Ni, Tiansen Li, Ke Zhang.
Abstract
Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.Entities:
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Year: 2014 PMID： 24994009 DOI： 10.1007/s12275-014-3689-9
Source DB: PubMed Journal: J Microbiol ISSN： 1225-8873 Impact factor: 3.422