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Literature DB >> 2057361

The chromosomal protein MC1 from the archaebacterium Methanosarcina sp. CHTI 55 induces DNA bending and supercoiling.

B Laine¹, F Culard, J C Maurizot, P Sautière.

Abstract

We have investigated the effect on the DNA structure of protein MC1, a basic and small polypeptide (Mr 10700) representing the major chromosomal protein in Methanosarcinaceae. The ability of protein MC1 to strongly favour cyclization upon polymerization of short DNA fragments by T4 DNA ligase indicates that protein MC1 mediates DNA bending. Several negatively supercoiled topoisomers of minicircles were obtained with DNA fragments of 203 and 146 bp, their distribution depends upon the amount of protein MC1 complexed with DNA. In addition, protein MC1 can induce a compaction of a nicked plasmid.

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Year: 1991 PMID： 2057361 PMCID： PMC328268 DOI： 10.1093/nar/19.11.3041

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. Conformational study of the chromosomal protein MC1 from the archaebacterium Methanosarcina barkeri.

Authors: M Imbert; B Laine; N Helbecque; J P Mornon; J P Hénichart; P Sautière
Journal: Biochim Biophys Acta Date: 1990-05-08

2. Primary structure of the chromosomal protein HMb from the archaebacteria Methanosarcina barkeri.

Authors: B Laine; F Chartier; M Imbert; R Lewis; P Sautiere
Journal: Eur J Biochem Date: 1986-12-15

Review 3. Histonelike proteins of bacteria.

Authors: K Drlica; J Rouviere-Yaniv
Journal: Microbiol Rev Date: 1987-09

4. Interaction of the Escherichia coli HU protein with DNA. Evidence for formation of nucleosome-like structures with altered DNA helical pitch.

Authors: S S Broyles; D E Pettijohn
Journal: J Mol Biol Date: 1986-01-05 Impact factor: 5.469

5. Characterization of the chromosomal protein MC1 from the thermophilic archaebacterium Methanosarcina sp. CHTI 55 and its effect on the thermal stability of DNA.

Authors: F Chartier; B Laine; P Sautiere
Journal: Biochim Biophys Acta Date: 1988-11-10

6. DNA flexibility studied by covalent closure of short fragments into circles.

Authors: D Shore; J Langowski; R L Baldwin
Journal: Proc Natl Acad Sci U S A Date: 1981-08 Impact factor: 11.205

7. Characterization and structural study of the DNA-binding protein HRm From Rhizobium meliloti.

Authors: B Laine; D Belaiche; P Sautiere; G Biserte
Journal: Biochem Biophys Res Commun Date: 1982-05-14 Impact factor: 3.575

8. E. coli DNA binding protein HU forms nucleosomelike structure with circular double-stranded DNA.

Authors: J Rouvière-Yaniv; M Yaniv; J E Germond
Journal: Cell Date: 1979-06 Impact factor: 41.582

9. The integration host factor of Escherichia coli binds to bent DNA at the origin of replication of the plasmid pSC101.

Authors: T T Stenzel; P Patel; D Bastia
Journal: Cell Date: 1987-06-05 Impact factor: 41.582

10. Variations of intramolecular ligation rates allow the detection of protein-induced bends in DNA.

Authors: D Kotlarz; A Fritsch; H Buc
Journal: EMBO J Date: 1986-04 Impact factor: 11.598

6 in total

Review 1. Do basic region-leucine zipper proteins bend their DNA targets ... does it matter?

Authors: P J Hagerman
Journal: Proc Natl Acad Sci U S A Date: 1996-09-17 Impact factor: 11.205

2. In vitro DNA binding of the archaeal protein Sso7d induces negative supercoiling at temperatures typical for thermophilic growth.

Authors: P López-García; S Knapp; R Ladenstein; P Forterre
Journal: Nucleic Acids Res Date: 1998-05-15 Impact factor: 16.971