Literature DB >> 20541518

A cautionary note on using N-acetylcysteine as an antagonist to assess isothiocyanate-induced reactive oxygen species-mediated apoptosis.

Lixin Mi1, Paul Sirajuddin, Nanqin Gan, Xiantao Wang.   

Abstract

N-Acetylcysteine (NAC) has been widely used in cell culture-based studies for the role of reactive oxygen species (ROS) generation in apoptosis induction by isothiocyanates (ITCs). Here we have demonstrated, using [(14)C]phenethyl ITC and [(14)C]sulforaphane, that NAC pretreatment significantly reduces ITC cellular uptake by conjugating with ITCs in the medium, suggesting that reduced uptake of ITCs, rather than the antioxidant activity of NAC itself, is responsible for the diminished downstream apoptotic effects. The study provides a cautionary note on the assay in studying mechanisms of apoptosis by ITCs and other electrophilic and thiol-reactive compounds. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20541518      PMCID： PMC2992988          DOI： 10.1016/j.ab.2010.06.015

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

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9.  Aggresome-like structure induced by isothiocyanates is novel proteasome-dependent degradation machinery.

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3.  Sulforaphane inhibits multiple inflammasomes through an Nrf2-independent mechanism.

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  8 in total

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