AIMS: To assess the degradation potential and plant colonization capacity of four alkane-degrading strains (ITSI10, ITRI15, ITRH76 and BTRH79) in combination with birdsfoot trefoil and Italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere. METHODS AND RESULTS: Contaminated soil was prepared by spiking agricultural soil with 10 g diesel fuel per kg soil. Italian ryegrass (Lolium multiflorum var. Taurus) and birdsfoot trefoil (Lotus corniculatus var. Leo) were inoculated with four alkane-degrading strains. Hydrocarbon degradation (up to 57%) was observed in all inoculated treatments of vegetated and unvegetated samples. Italian ryegrass in combination with compost and BTRH79 showed highest degradation, while birdsfoot trefoil performed best with compost and strain ITSI10. Cultivation-based as well as cultivation-independent analysis showed that both strains were competitive colonizers. CONCLUSIONS: The combination between vegetation, inoculation with well-performing degrading bacteria and compost amendment was an efficient approach to reduce hydrocarbon contamination. Two Pantoea sp. strains, ITSI10 and BTRH79, established well in the plant environment despite the presence of a variety of other, indigenous alkane-degrading bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: This study suggests that the application of degrading bacterial strains, which are able to compete with the native microflora and to tightly associate with plants, are promising candidates to be used for phytoremediation applications.
AIMS: To assess the degradation potential and plant colonization capacity of four alkane-degrading strains (ITSI10, ITRI15, ITRH76 and BTRH79) in combination with birdsfoot trefoil and Italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere. METHODS AND RESULTS: Contaminated soil was prepared by spiking agricultural soil with 10 g diesel fuel per kg soil. Italian ryegrass (Lolium multiflorum var. Taurus) and birdsfoot trefoil (Lotus corniculatus var. Leo) were inoculated with four alkane-degrading strains. Hydrocarbon degradation (up to 57%) was observed in all inoculated treatments of vegetated and unvegetated samples. Italian ryegrass in combination with compost and BTRH79 showed highest degradation, while birdsfoot trefoil performed best with compost and strain ITSI10. Cultivation-based as well as cultivation-independent analysis showed that both strains were competitive colonizers. CONCLUSIONS: The combination between vegetation, inoculation with well-performing degrading bacteria and compost amendment was an efficient approach to reduce hydrocarbon contamination. Two Pantoea sp. strains, ITSI10 and BTRH79, established well in the plant environment despite the presence of a variety of other, indigenous alkane-degrading bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: This study suggests that the application of degrading bacterial strains, which are able to compete with the native microflora and to tightly associate with plants, are promising candidates to be used for phytoremediation applications.
Authors: Habib-Ur-Rehman Athar; Sarah Ambreen; Muhammad Javed; Mehwish Hina; Sumaira Rasul; Zafar Ullah Zafar; Hamid Manzoor; Chukwuma C Ogbaga; Muhammad Afzal; Fahad Al-Qurainy; Muhammad Ashraf Journal: Environ Sci Pollut Res Int Date: 2016-06-08 Impact factor: 4.223
Authors: Vanessa Oliveira; Newton C M Gomes; Adelaide Almeida; Artur M S Silva; Helena Silva; Ângela Cunha Journal: Microb Ecol Date: 2014-07-08 Impact factor: 4.552
Authors: Muhammad U Khan; Angela Sessitsch; Muhammad Harris; Kaneez Fatima; Asma Imran; Muhammad Arslan; Ghulam Shabir; Qaiser M Khan; Muhammad Afzal Journal: Front Plant Sci Date: 2015-01-06 Impact factor: 5.753
Authors: Panagiotis Gkorezis; Matteo Daghio; Andrea Franzetti; Jonathan D Van Hamme; Wouter Sillen; Jaco Vangronsveld Journal: Front Microbiol Date: 2016-11-21 Impact factor: 5.640