Literature DB >> 20520890

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution.

Weng Lin Tang1, Zhi Li, Huimin Zhao.   

Abstract

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

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Year:  2010        PMID： 20520890     DOI： 10.1039/c0cc00735h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  8 in total

Review 1.  Biocatalyst development by directed evolution.

Authors:  Meng Wang; Tong Si; Huimin Zhao
Journal:  Bioresour Technol       Date:  2012-01-21       Impact factor: 9.642

2.  A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts.

Authors:  Tristan de Rond; Jian Gao; Amin Zargar; Markus de Raad; Jack Cunha; Trent R Northen; Jay D Keasling
Journal:  Angew Chem Int Ed Engl       Date:  2019-06-13       Impact factor: 15.336

Review 3.  Cytochrome P450: taming a wild type enzyme.

Authors:  Sang Taek Jung; Ryan Lauchli; Frances H Arnold
Journal:  Curr Opin Biotechnol       Date:  2011-03-14       Impact factor: 9.740

Review 4.  Oxygenating Biocatalysts for Hydroxyl Functionalisation in Drug Discovery and Development.

Authors:  Sacha N Charlton; Martin A Hayes
Journal:  ChemMedChem       Date:  2022-05-02       Impact factor: 3.540

5.  Comprehensive Structure-Activity Profiling of Micheliolide and its Targeted Proteome in Leukemia Cells via Probe-Guided Late-Stage C-H Functionalization.

Authors:  Hanan Alwaseem; Simone Giovani; Michele Crotti; Kevin Welle; Craig T Jordan; Sina Ghaemmaghami; Rudi Fasan
Journal:  ACS Cent Sci       Date:  2021-04-28       Impact factor: 14.553

6.  Controlled oxidation of remote sp3 C-H bonds in artemisinin via P450 catalysts with fine-tuned regio- and stereoselectivity.

Authors:  Kaidong Zhang; Brian M Shafer; Matthew D Demars; Harry A Stern; Rudi Fasan
Journal:  J Am Chem Soc       Date:  2012-11-02       Impact factor: 15.419

7.  The "gate keeper" role of Trp222 determines the enantiopreference of diketoreductase toward 2-chloro-1-phenylethanone.

Authors:  Hairong Ma; Xin Yang; Zhuo Lu; Nan Liu; Yijun Chen
Journal:  PLoS One       Date:  2014-07-29       Impact factor: 3.240

8.  A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3.

Authors:  Anna Gärtner; Anna Joëlle Ruff; Ulrich Schwaneberg
Journal:  Sci Rep       Date:  2019-10-29       Impact factor: 4.379
  8 in total

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