Literature DB >> 20516600

Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter.

Andreas Zurbriggen1, Philipp Schneider, Priska Bähler, Ulrich Baumann, Bernhard Erni.   

Abstract

The glucose-import system of Escherichia coli consists of a hydrophilic EIIA(Glc) subunit and a transmembrane EIICB(Glc) subunit. EIICB(Glc) (UniProt P69786) contains two domains: the transmembrane EIIC(Glc) domain (40.6 kDa) and the cytoplasmic EIIB(Glc) domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICB(Glc) subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIIC(Glc) domain is described. A complete data set was collected to 4.5 A resolution at 100 K.

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Year:  2010        PMID： 20516600      PMCID： PMC2882770          DOI： 10.1107/S1744309110013102

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


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