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Literature DB >> 20513154

Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.

Ryuji Igarashi¹, Tomomi Sakai, Hideyuki Hara, Takeshi Tenno, Toshiaki Tanaka, Hidehito Tochio, Masahiro Shirakawa.

Abstract

DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.

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Year: 2010 PMID： 20513154 DOI： 10.1021/ja906104e

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

24 in total

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3. Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy.

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Journal: Angew Chem Int Ed Engl Date: 2015-03-31 Impact factor: 15.336

Distance determination in proteins inside Xenopus laevis oocytes by double electron-electron resonance experiments.

1. In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells.

2. Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment.

3. Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy.

Review 4. New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.

5. PELDOR/DEER: An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers.

6. Ligand Induced Conformational Changes of a Membrane Transporter in E. coli Cells Observed with DEER/PELDOR.

7. In-cell destabilization of a homodimeric protein complex detected by DEER spectroscopy.

8. Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy.

9. Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance Measurements In-Cell.

10. In Situ Detection of Endogenous HIV Activation by Dynamic Nuclear Polarization NMR and Flow Cytometry.