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Literature DB >> 20493852

A tRNA-dependent cysteine biosynthesis enzyme recognizes the selenocysteine-specific tRNA in Escherichia coli.

Jing Yuan¹, Michael J Hohn, R Lynn Sherrer, Sotiria Palioura, Dan Su, Dieter Söll.

Abstract

The essential methanogen enzyme Sep-tRNA:Cys-tRNA synthase (SepCysS) converts O-phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)) into Cys-tRNA(Cys) in the presence of a sulfur donor. Likewise, Sep-tRNA:Sec-tRNA synthase converts O-phosphoseryl-tRNA(Sec) (Sep-tRNA(Sec)) to selenocysteinyl-tRNA(Sec) (Sec-tRNA(Sec)) using a selenium donor. While the Sep moiety of the aminoacyl-tRNA substrates is the same in both reactions, tRNA(Cys) and tRNA(Sec) differ greatly in sequence and structure. In an Escherichia coli genetic approach that tests for formate dehydrogenase activity in the absence of selenium donor we show that Sep-tRNA(Sec) is a substrate for SepCysS. Since Sec and Cys are the only active site amino acids known to sustain FDH activity, we conclude that SepCysS converts Sep-tRNA(Sec) to Cys-tRNA(Sec), and that Sep is crucial for SepCysS recognition. Copyright 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year: 2010 PMID： 20493852 PMCID： PMC2893348 DOI： 10.1016/j.febslet.2010.05.028

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

32 in total

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Authors: Jing Yuan; Patrick O'Donoghue; Alex Ambrogelly; Sarath Gundllapalli; R Lynn Sherrer; Sotiria Palioura; Miljan Simonović; Dieter Söll
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12 in total

1. Mutational analysis of Sep-tRNA:Cys-tRNA synthase reveals critical residues for tRNA-dependent cysteine formation.

Authors: Sunna Helgadóttir; Sylvie Sinapah; Dieter Söll; Jiqiang Ling
Journal: FEBS Lett Date: 2011-12-09 Impact factor: 4.124

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4. Random mutagenesis identifies factors involved in formate-dependent growth of the methanogenic archaeon Methanococcus maripaludis.

Authors: Christian Sattler; Sandro Wolf; Julia Fersch; Stefan Goetz; Michael Rother
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5. Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis.

Authors: Michael J Hohn; Sotiria Palioura; Dan Su; Jing Yuan; Dieter Söll
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Authors: Yuzuru Itoh; Markus J Bröcker; Shun-ichi Sekine; Dieter Söll; Shigeyuki Yokoyama
Journal: J Mol Biol Date: 2014-01-20 Impact factor: 5.469