OBJECTIVE: The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC). STUDY DESIGN: Cross-sectional study. SETTING: University-based medical center. SUBJECTS AND METHODS: Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337 nm, 700-picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time-domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined. RESULTS: Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660 nm. Multiple time-resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390 nm: 1.7 +/- 0.06 ns [not significant] for normal and 1.3 +/- 0.06 ns for tumor, P = 0.0025) and the second-order Laguerre expansion coefficient (LEC-2) (i.e., at 460 nm: 0.135 +/- 0.001 for normal and 0.155 +/- 0.007 for tumor, P < 0.05). CONCLUSION: These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR-LIFS has potential as a noninvasive diagnostic technique for HNSCC. Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved.
OBJECTIVE: The objectives of this study were to 1) determine differences in lifetime fluorescence between normal and malignant tissue of the upper aerodigestive tract, and 2) evaluate the potential of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) as a diagnostic instrument for head and neck squamous cell carcinoma (HNSCC). STUDY DESIGN: Cross-sectional study. SETTING: University-based medical center. SUBJECTS AND METHODS: Nine patients with suspected HNSCC were included. In the operating room, a nitrogen pulse laser (337 nm, 700-picosecond pulse width) was used to induce tissue autofluorescence of normal tissue and suspected malignant lesions. Spectral intensities and time-domain measurements were obtained and compared with the histopathology at each site. A total of 53 sites were measured. The fluorescence parameters that provided the most discrimination were determined. RESULTS: Differences in spectral intensities allowed for discrimination between malignant and normal tissue. The spectral intensity of malignant tissue was lower than that of normal tissue, and a shift of peak intensity to a longer wavelength was observed in the normalized spectrum of malignant tissue in the range of 360 to approximately 660 nm. Multiple time-resolved fluorescence parameters provided the best diagnostic discrimination between normal tissue and carcinoma, including average lifetimes (i.e., at 390 nm: 1.7 +/- 0.06 ns [not significant] for normal and 1.3 +/- 0.06 ns for tumor, P = 0.0025) and the second-order Laguerre expansion coefficient (LEC-2) (i.e., at 460 nm: 0.135 +/- 0.001 for normal and 0.155 +/- 0.007 for tumor, P < 0.05). CONCLUSION: These findings highlight some of the differences in lifetime fluorescence between normal and malignant tissue. TR-LIFS has potential as a noninvasive diagnostic technique for HNSCC. Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved.
Authors: Kamran Badizadegan; Vadim Backman; Charles W Boone; Christopher P Crum; Ramachandra R Dasari; Irene Georgakoudi; Kristin Keefe; Karl Munger; Stanley M Shapshay; Ellen E Sheetse; Michael S Feld Journal: Faraday Discuss Date: 2004 Impact factor: 4.008
Authors: Richard A Schwarz; Wen Gao; Dania Daye; Michelle D Williams; Rebecca Richards-Kortum; Ann M Gillenwater Journal: Appl Opt Date: 2008-02-20 Impact factor: 1.980
Authors: D L Heintzelman; U Utzinger; H Fuchs; A Zuluaga; K Gossage; A M Gillenwater; R Jacob; B Kemp; R R Richards-Kortum Journal: Photochem Photobiol Date: 2000-07 Impact factor: 3.421
Authors: D Gregory Farwell; Jeremy D Meier; Jesung Park; Yang Sun; Heather Coffman; Brian Poirier; Jennifer Phipps; Steve Tinling; Danny J Enepekides; Laura Marcu Journal: Arch Otolaryngol Head Neck Surg Date: 2010-02
Authors: Laura Marcu; Qiyin Fang; Javier A Jo; Thanassis Papaioannou; Amir Dorafshar; Todd Reil; Jian-Hua Qiao; J Dennis Baker; Julie A Freischlag; Michael C Fishbein Journal: Atherosclerosis Date: 2005-04-02 Impact factor: 5.162
Authors: A Gillenwater; R Jacob; R Ganeshappa; B Kemp; A K El-Naggar; J L Palmer; G Clayman; M F Mitchell; R Richards-Kortum Journal: Arch Otolaryngol Head Neck Surg Date: 1998-11
Authors: Lisa M Mahakian; D Gregory Farwell; Hua Zhang; Jai Woong Seo; Brian Poirier; Steven P Tinling; Alaa M Afify; Eric M Haynam; David Shaye; Katherine W Ferrara Journal: Mol Imaging Biol Date: 2014-04 Impact factor: 3.488
Authors: Mary Roz Timbang; Michael W Sim; Arnaud F Bewley; D Gregory Farwell; Avinash Mantravadi; Michael G Moore Journal: Hum Vaccin Immunother Date: 2019-05-07 Impact factor: 3.452
Authors: Hussain Fatakdawala; Shannon Poti; Feifei Zhou; Yang Sun; Julien Bec; Jing Liu; Diego R Yankelevich; Steven P Tinling; Regina F Gandour-Edwards; D Gregory Farwell; Laura Marcu Journal: Biomed Opt Express Date: 2013-08-26 Impact factor: 3.732
Authors: Elvis Duran-Sierra; Shuna Cheng; Rodrigo Cuenca-Martinez; Bilal Malik; Kristen C Maitland; Y S Lisa Cheng; John Wright; Beena Ahmed; Jim Ji; Mathias Martinez; Moustafa Al-Khalil; Hussain Al-Enazi; Javier A Jo Journal: Oral Oncol Date: 2020-04-02 Impact factor: 5.337
Authors: Yang Sun; Hongtao Xie; Jing Liu; Matthew Lam; Abhijit J Chaudhari; Feifei Zhou; Julien Bec; Diego R Yankelevich; Allison Dobbie; Steven L Tinling; Regina F Gandour-Edwards; Wayne L Monsky; D Gregory Farwell; Laura Marcu Journal: J Biomed Opt Date: 2012-11 Impact factor: 3.170