Literature DB >> 20485474

Enhanced Single-Molecule Detection using Porous Silver Membrane.

Yi Fu1, Joseph R Lakowicz.   

Abstract

We evaluated a commercial porous silver membrane as a support substrate for direct localization and visualization of single molecule events. We characterized the fluorescence behaviors of fluorescently labeled DNA oligonucleotides on the silver membranes. The fluorescence from the fluorescent probes that were immobilized on the porous silver is greatly enhanced. Additionally, correlated to reflectance contour image, it appears that enhanced fluorescence came from location close to the "valley" of the pore channels (or in the voids). These results are of great interest to increase the effectiveness of fluorescence-based single molecule DNA analysis.

Entities:  

Year:  2010        PMID： 20485474      PMCID： PMC2871712          DOI： 10.1021/jp911407c

Source DB:  PubMed          Journal:  J Phys Chem C Nanomater Interfaces        ISSN： 1932-7447            Impact factor:   4.126


  29 in total

1.  A theoretical investigation of single-molecule fluorescence detection on thin metallic layers.

Authors:  J Enderlein
Journal:  Biophys J       Date:  2000-04       Impact factor: 4.033

2.  Single-molecule detection of DNA hybridization.

Authors:  Mukta Singh-Zocchi; Sanhita Dixit; Vassili Ivanov; Giovanni Zocchi
Journal:  Proc Natl Acad Sci U S A       Date:  2003-06-13       Impact factor: 11.205

3.  Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence images and time-resolved studies using single-molecule spectroscopy.

Authors:  Yi Fu; Joseph R Lakowicz
Journal:  Anal Chem       Date:  2006-09-01       Impact factor: 6.986

Review 4.  Toward nanoscale genome sequencing.

Authors:  Declan Ryan; Maryam Rahimi; John Lund; Ranjana Mehta; Babak A Parviz
Journal:  Trends Biotechnol       Date:  2007-07-19       Impact factor: 19.536

Review 5.  Nanopore-based single-molecule DNA analysis.

Authors:  Ken Healy
Journal:  Nanomedicine (Lond)       Date:  2007-08       Impact factor: 5.307

6.  Plasmonics in Biology and Plasmon-Controlled Fluorescence.

Authors:  Joseph R Lakowicz
Journal:  Plasmonics       Date:  2006-03-01       Impact factor: 2.404

Review 7.  Exploring the new world of the genome with DNA microarrays.

Authors:  P O Brown; D Botstein
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

8.  Toward efficient nanoporous catalysts: controlling site-isolation and concentration of grafted catalytic sites on nanoporous materials with solvents and colorimetric elucidation of their site-isolation.

Authors:  Krishna K Sharma; Abhishek Anan; Robert P Buckley; Wayne Ouellette; Tewodros Asefa
Journal:  J Am Chem Soc       Date:  2007-12-13       Impact factor: 15.419

Review 9.  Optical probing of single fluorescent molecules and proteins.

Authors:  M F García-Parajó; J A Veerman; R Bouwhuis; R Vallée; N F van Hulst
Journal:  Chemphyschem       Date:  2001-06-18       Impact factor: 3.102

Review 10.  The potential and challenges of nanopore sequencing.

Authors:  Daniel Branton; David W Deamer; Andre Marziali; Hagan Bayley; Steven A Benner; Thomas Butler; Massimiliano Di Ventra; Slaven Garaj; Andrew Hibbs; Xiaohua Huang; Stevan B Jovanovich; Predrag S Krstic; Stuart Lindsay; Xinsheng Sean Ling; Carlos H Mastrangelo; Amit Meller; John S Oliver; Yuriy V Pershin; J Michael Ramsey; Robert Riehn; Gautam V Soni; Vincent Tabard-Cossa; Meni Wanunu; Matthew Wiggin; Jeffery A Schloss
Journal:  Nat Biotechnol       Date:  2008-10       Impact factor: 54.908
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  1 in total

1.  Metallic-Nanostructure-Enhanced Fluorescence of Single Flavin Cofactor and Single Flavoenzyme Molecules.

Authors:  Yi Fu; Jian Zhang; Joseph R Lakowicz
Journal:  J Phys Chem C Nanomater Interfaces       Date:  2011-03-24       Impact factor: 4.126
  1 in total

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