| Literature DB >> 20471595 |
Nobuhiko Mukai1, Kazuo Masaki, Tsutomu Fujii, Makoto Kawamukai, Haruyuki Iefuji.
Abstract
The volatile phenols, to which Saccharomyces cerevisiae converts from phenylacrylic acids including ferulic acid, p-coumaric acid, and cinnamic acid, generate off-flavors in alcoholic beverages such as beer and wine. Using gene disruptants, transformants and cell-free extracts of these strains, we have verified that the adjacent PAD1 (phenylacrylic acid decarboxylase, YDR538W) and FDC1 (ferulic acid decarboxylase, YDR539W) genes are essential for the decarboxylation of phenylacrylic acids in S. cerevisiae. Pad1p and Fdc1p are homologous with UbiX and UbiD, respectively, in the ubiquinone synthetic pathway of Escherichia coli. However, ubiquinone was detected quantitatively in all of the yeast single-deletion mutants, Delta pad1, Delta fdc1, and double-deletion mutant, Delta pad1 Delta fdc1. (c) 2009 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.Entities:
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Year: 2009 PMID： 20471595 DOI： 10.1016/j.jbiosc.2009.11.011
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894