Literature DB >> 20445243

Structure of orotate phosphoribosyltransferase from the caries pathogen Streptococcus mutans.

Chao Pei Liu1, Rui Xu, Zeng Qiang Gao, Jian Hua Xu, Hai Feng Hou, Li Qin Li, Zhun She, Lan Fen Li, Xiao Dong Su, Peng Liu, Yu Hui Dong.   

Abstract

Orotate phosphoribosyltransferase (OPRTase) catalyzes the OMP-forming step in de novo pyrimidine-nucleotide biosynthesis. Here, the crystal structure of OPRTase from the caries pathogen Streptococcus mutans is reported at 2.4 A resolution. S. mutans OPRTase forms a symmetric dimer and each monomer binds two sulfates at the active sites. The structural symmetry of the sulfate-binding sites and the missing loops in this structure are consistent with a symmetric catalysis mechanism.

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Year:  2010        PMID： 20445243      PMCID： PMC2864676          DOI： 10.1107/S1744309110009243

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  22 in total

1.  Efficient anisotropic refinement of macromolecular structures using FFT.

Authors:  G N Murshudov; A A Vagin; A Lebedev; K S Wilson; E J Dodson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

2.  Refinement of macromolecular structures by the maximum-likelihood method.

Authors:  G N Murshudov; A A Vagin; E J Dodson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1997-05-01

3.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

4.  Likelihood-enhanced fast rotation functions.

Authors:  Laurent C Storoni; Airlie J McCoy; Randy J Read
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-02-25

5.  Studies of the kinetic mechanism of orotate phosphoribosyltransferase from yeast.

Authors:  J Victor; L B Greenberg; D L Sloan
Journal:  J Biol Chem       Date:  1979-04-25       Impact factor: 5.157

6.  Coot: model-building tools for molecular graphics.

Authors:  Paul Emsley; Kevin Cowtan
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-11-26

7.  Likelihood-enhanced fast translation functions.

Authors:  Airlie J McCoy; Ralf W Grosse-Kunstleve; Laurent C Storoni; Randy J Read
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-03-24

8.  Version 1.2 of the Crystallography and NMR system.

Authors:  Axel T Brunger
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

9.  Half-of-sites binding of orotidine 5'-phosphate and alpha-D-5-phosphorylribose 1-diphosphate to orotate phosphoribosyltransferase from Saccharomyces cerevisiae supports a novel variant of the Theorell-Chance mechanism with alternating site catalysis.

Authors:  Ronald W McClard; Edward A Holets; Andrew L MacKinnon; John F Witte
Journal:  Biochemistry       Date:  2006-04-25       Impact factor: 3.162

10.  Ternary complex formation and induced asymmetry in orotate phosphoribosyltransferase.

Authors:  Lilian González-Segura; John F Witte; Ronald W McClard; Thomas D Hurley
Journal:  Biochemistry       Date:  2007-11-20       Impact factor: 3.162
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  4 in total

1.  Crystallization and preliminary X-ray diffraction analysis of orotate phosphoribosyltransferase from the human malaria parasite Plasmodium falciparum.

Authors:  Yasuhide Takashima; Eiichi Mizohata; Keiji Tokuoka; Sudaratana R Krungkrai; Yukiko Kusakari; Saki Konishi; Atsuko Satoh; Hiroyoshi Matsumura; Jerapan Krungkrai; Toshihiro Horii; Tsuyoshi Inoue
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-01-27

Review 2.  Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.

Authors:  Bjarne Hove-Jensen; Kasper R Andersen; Mogens Kilstrup; Jan Martinussen; Robert L Switzer; Martin Willemoës
Journal:  Microbiol Mol Biol Rev       Date:  2016-12-28       Impact factor: 11.056

3.  Structure of Plasmodium falciparum orotate phosphoribosyltransferase with autologous inhibitory protein-protein interactions.

Authors:  Shiva Kumar; Kalyanaraman Krishnamoorthy; Devaraja G Mudeppa; Pradipsinh K Rathod
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-04-21       Impact factor: 1.056

4.  Structural investigations on orotate phosphoribosyltransferase from Mycobacterium tuberculosis, a key enzyme of the de novo pyrimidine biosynthesis.

Authors:  Stefano Donini; Davide M Ferraris; Riccardo Miggiano; Alberto Massarotti; Menico Rizzi
Journal:  Sci Rep       Date:  2017-04-26       Impact factor: 4.379
  4 in total

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