Titration of herpes simplex virus antibodies in human sera by the enzyme-linked immunosorbent assay (ELISA).

100 sera from healthy adults were titrated simultaneously for herpes simplex virus (HSV) antibodies by ELISA and neutralization. The ELISA was performed on microtitre plates where approximately 100 ng of detergent solubilized and chromatographically purified HSV glycoproteins was bound covalently to the plastic bottom of each well. The optical density (OD) values obtained by the use of the peroxidase-1.2phenylendiamindihydrochloride system showed good correlation with the neuratlizing antibody titres. Sera with very low neutralizing titres were clearly positive in ELISA.