Literature DB >> 6244329

Enzyme immunoassay for measurement of antibodies to herpes simplex virus infection: comparison with complement fixation, immunofluorescent-antibody, and neutralization techniques.

G A Denoyel, A Gaspar, C Nouyrigat.   

Abstract

An enzyme immunoassay (EIA) was developed for detecting antibody to herpes simplex virus, and the results were compared with those of complement fixation, indirect immunofluorescent-antibody, and plaque reduction neutralization tests. Test sera showed very little nonspecific reactivity even at a starting dilution as low as 1:10. EIA results showed excellent correlation with results obtained by the neutralization test, with an average gain in sensitivity of 1.65. EIA proved very useful in detecting current herpes simplex virus infection, and antibody appeared in all cases soon after clinical onset. EIA appears to be a rapid, sensitive, and specific method for routine demonstration of herpes simplex virus antibody in a clinical setting.

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Year:  1980        PMID： 6244329      PMCID： PMC273334          DOI： 10.1128/jcm.11.2.114-119.1980

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes.

Authors:  E Engvall; K Jonsson; P Perlmann
Journal:  Biochim Biophys Acta       Date:  1971-12-28

2.  Application of a microtechnique to viral serological investigations.

Authors:  J L SEVER
Journal:  J Immunol       Date:  1962-03       Impact factor: 5.422

3.  Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites.

Authors:  G S Hayward; N Frenkel; B Roizman
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

4.  IgG, IgA and IgM antibody responses in patients with acute genital and non-genital herpetic lesions determined by the indirect fluorescent antibody method.

Authors:  I H El Falaky; B F Vestergaard; A Hornsleth
Journal:  Scand J Infect Dis       Date:  1977

5.  Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays.

Authors:  E J Ruitenberg; P A Steerenberg; B J Brosi; J Buys
Journal:  Bull World Health Organ       Date:  1974       Impact factor: 9.408

Review 6.  Antigens of herpes simplex virus of oral and genital origin.

Authors:  P Wildy
Journal:  Cancer Res       Date:  1973-06       Impact factor: 12.701

7.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

8.  Immunological relationship between herpes simplex and varicella-zoster viruses demonstrated by complement-fixation, neutralization and fluorescent antibody tests.

Authors:  N J Schmidt; E H Lennette; R L Magoffin
Journal:  J Gen Virol       Date:  1969-04       Impact factor: 3.891

9.  The incidence of Herpesvirus hominis antibody in the population.

Authors:  I W Smith; J F Peutherer; F O MacCallum
Journal:  J Hyg (Lond)       Date:  1967-09

10.  Inconstant ELISA.

Authors:  B S Chessum; J R Denmark
Journal:  Lancet       Date:  1978-01-21       Impact factor: 79.321
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  8 in total

1.  Freeze-dried erythrocytes for an indirect hemagglutination test for detection of cytomegalovirus antibodies.

Authors:  N Cabau; R Crainic; C Duros; G Denoyel; A Gaspar; C Bronnert; A Boué; F Horodniceanu
Journal:  J Clin Microbiol       Date:  1981-06       Impact factor: 5.948

2.  Development of an enzyme-linked immunosorbent assay for equine infectious anemia virus detection using recombinant Pr55gag.

Authors:  D Archambault; Z M Wang; J C Lacal; A Gazit; A Yaniv; J E Dahlberg; S R Tronick
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

3.  Rapid diagnosis of ocular herpes simplex infections.

Authors:  P A Asbell; M A Torres; T Kamenar; E J Bottone
Journal:  Br J Ophthalmol       Date:  1995-05       Impact factor: 4.638

4.  Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors.

Authors:  H Moser; F Behrens; R Ziegelmaier; J Hilfenhaus; R Mauler
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

5.  Evaluation of the polymerase chain reaction for diagnosis of herpes simplex virus encephalitis.

Authors:  E Puchhammer-Stöckl; F X Heinz; M Kundi; T Popow-Kraupp; G Grimm; M M Millner; C Kunz
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948

6.  Development of an enzyme-linked immunosorbent assay for caprine arthritis-encephalitis virus.

Authors:  D Archambault; N East; K Perk; J E Dahlberg
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948

7.  Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.

Authors:  R M Coleman; L Pereira; P D Bailey; D Dondero; C Wickliffe; A J Nahmias
Journal:  J Clin Microbiol       Date:  1983-08       Impact factor: 5.948

8.  Evaluation of a computer-assisted, kinetics-based enzyme-linked immunosorbent assay for detection of coronavirus antibodies in cats.

Authors:  J E Barlough; R H Jacobson; D R Downing; K L Marcella; T J Lynch; F W Scott
Journal:  J Clin Microbiol       Date:  1983-02       Impact factor: 5.948
  8 in total

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