BACKGROUND: Recent technological advances in miniaturisation have allowed for a confocal scanning microscope to be integrated into trans-endoscopic probes enabling endoscopists to collect in vivo virtual biopsies of the gastrointestinal mucosa during endoscopy. AIMS: The aim of the present study was to assess prospectively the clinical applicability and predictive power of a probe-based confocal laser endomicroscopy for the in vivo diagnosis of colorectal neoplasia. METHODS: Patients with evidence of colorectal superficial neoplasia at routine endoscopy, were included prospectively in this study. Lesions were identified using white-light endoscopy followed by pCLE imaging recorded by a Coloflex UHD-type probe. The images were interpreted as either neoplastic or not according to vascular and cellular changes. pCLE readings were then compared with histopathological results from endoscopically resected lesions and/or targeted biopsy specimens. RESULTS: A total of 32 lesions were identified in 20 consecutive patients. Histopathology diagnosis was of adenomas in 19 cases, hyperplastic polyps in 11 cases and adenocarcinoma in 2 cases. For the detection of neoplastic tissue pCLE had a sensitivity of 100%, a specificity of 84.6%, an accuracy of 92.3, a PPV of 90.5% and a NPV of 100%. CONCLUSIONS: pCLE permits high-quality imaging, enabling prediction of intraepithelial neoplasia with a high level of accuracy.
BACKGROUND: Recent technological advances in miniaturisation have allowed for a confocal scanning microscope to be integrated into trans-endoscopic probes enabling endoscopists to collect in vivo virtual biopsies of the gastrointestinal mucosa during endoscopy. AIMS: The aim of the present study was to assess prospectively the clinical applicability and predictive power of a probe-based confocal laser endomicroscopy for the in vivo diagnosis of colorectal neoplasia. METHODS:Patients with evidence of colorectal superficial neoplasia at routine endoscopy, were included prospectively in this study. Lesions were identified using white-light endoscopy followed by pCLE imaging recorded by a Coloflex UHD-type probe. The images were interpreted as either neoplastic or not according to vascular and cellular changes. pCLE readings were then compared with histopathological results from endoscopically resected lesions and/or targeted biopsy specimens. RESULTS: A total of 32 lesions were identified in 20 consecutive patients. Histopathology diagnosis was of adenomas in 19 cases, hyperplastic polyps in 11 cases and adenocarcinoma in 2 cases. For the detection of neoplastic tissue pCLE had a sensitivity of 100%, a specificity of 84.6%, an accuracy of 92.3, a PPV of 90.5% and a NPV of 100%. CONCLUSIONS: pCLE permits high-quality imaging, enabling prediction of intraepithelial neoplasia with a high level of accuracy.
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Authors: Antonio Rispo; Fabiana Castiglione; Stefania Staibano; Dario Esposito; Francesco Maione; Maria Siano; Francesca Salvatori; Stefania Masone; Marcello Persico; Giovanni Domenico De Palma Journal: World J Gastrointest Endosc Date: 2012-09-16
Authors: Bun Kim; Yon Hee Kim; Soo Jung Park; Jae Hee Cheon; Tae Il Kim; Won Ho Kim; Hoguen Kim; Sung Pil Hong Journal: Surg Endosc Date: 2016-06-20 Impact factor: 4.584
Authors: Giovanni D De Palma; Gaetano Luglio; Stefania Staibano; Luigi Bucci; Dario Esposito; Francesco Maione; Massimo Mascolo; Gennaro Ilardi; Pietro Forestieri Journal: Surg Endosc Date: 2014-02-12 Impact factor: 4.584