OBJECTIVES: To develop the diagnostic criteria for benign and neoplastic conditions of the urinary tract using probe-based confocal laser endomicroscopy (pCLE), a new technology for dynamic, in vivo imaging with micron-scale resolution. The suggested diagnostic criteria will formulate a guide for pCLE image interpretation in urology. METHODS: Patients scheduled for transurethral resection of bladder tumor (TURBT) or nephrectomy were recruited. After white-light cystoscopy (WLC), fluorescein was administered as contrast. Different areas of the urinary tract were imaged with pCLE via direct contact between the confocal probe and the area of interest. Confocal images were subsequently compared with standard hematoxylin and eosin analysis. RESULTS: pCLE images were collected from 66 participants, including 2 patients who underwent nephrectomy. We identified key features associated with different anatomic landmarks of the urinary tract, including the kidney, ureter, bladder, prostate, and urethra. In vivo pCLE of the bladder demonstrated distinct differences between normal mucosa and neoplastic tissue. Using mosaicing, a post hoc image-processing algorithm, individual image frames were juxtaposed to form wide-angle views to better evaluate tissue microarchitecture. CONCLUSIONS: In contrast to standard pathologic analysis of fixed tissue with hematoxylin and eosin, pCLE provides real time microscopy of the urinary tract to enable dynamic interrogation of benign and neoplastic tissues in vivo. The diagnostic criteria developed in this study will facilitate adaptation of pCLE for use in conjunction with WLC to expedite diagnosis of urinary tract pathology, particularly bladder cancer. Published by Elsevier Inc.
OBJECTIVES: To develop the diagnostic criteria for benign and neoplastic conditions of the urinary tract using probe-based confocal laser endomicroscopy (pCLE), a new technology for dynamic, in vivo imaging with micron-scale resolution. The suggested diagnostic criteria will formulate a guide for pCLE image interpretation in urology. METHODS:Patients scheduled for transurethral resection of bladder tumor (TURBT) or nephrectomy were recruited. After white-light cystoscopy (WLC), fluorescein was administered as contrast. Different areas of the urinary tract were imaged with pCLE via direct contact between the confocal probe and the area of interest. Confocal images were subsequently compared with standard hematoxylin and eosin analysis. RESULTS:pCLE images were collected from 66 participants, including 2 patients who underwent nephrectomy. We identified key features associated with different anatomic landmarks of the urinary tract, including the kidney, ureter, bladder, prostate, and urethra. In vivo pCLE of the bladder demonstrated distinct differences between normal mucosa and neoplastic tissue. Using mosaicing, a post hoc image-processing algorithm, individual image frames were juxtaposed to form wide-angle views to better evaluate tissue microarchitecture. CONCLUSIONS: In contrast to standard pathologic analysis of fixed tissue with hematoxylin and eosin, pCLE provides real time microscopy of the urinary tract to enable dynamic interrogation of benign and neoplastic tissues in vivo. The diagnostic criteria developed in this study will facilitate adaptation of pCLE for use in conjunction with WLC to expedite diagnosis of urinary tract pathology, particularly bladder cancer. Published by Elsevier Inc.
Authors: Alice Rickard; Nikolay Dorokhov; Jan Ryerse; David J Klumpp; Jane McHowat Journal: In Vitro Cell Dev Biol Anim Date: 2008-06-14 Impact factor: 2.416
Authors: Winifred Adams; Katherine Wu; Jen-Jane Liu; Shelly Teng Teng Hsiao; Kristin C Jensen; Joseph C Liao Journal: J Endourol Date: 2011-05-13 Impact factor: 2.942
Authors: Valentin Becker; Tom Vercauteren; Claus Hann von Weyhern; Christian Prinz; Roland M Schmid; Alexander Meining Journal: Gastrointest Endosc Date: 2007-09-04 Impact factor: 9.427
Authors: Pei-Lin Hsiung; Pei-Lei Hsiung; Jonathan Hardy; Shai Friedland; Roy Soetikno; Christine B Du; Amy P Wu; Peyman Sahbaie; James M Crawford; Anson W Lowe; Christopher H Contag; Thomas D Wang Journal: Nat Med Date: 2008-03-16 Impact factor: 53.440
Authors: Thomas D Wang; Shai Friedland; Peyman Sahbaie; Roy Soetikno; Pei-Lin Hsiung; Jonathan T C Liu; James M Crawford; Christopher H Contag Journal: Clin Gastroenterol Hepatol Date: 2007-10-23 Impact factor: 11.382
Authors: Geoffrey A Sonn; Kathleen E Mach; Kristin Jensen; Pei-Lin Hsiung; Sha-Nita Jones; Christopher H Contag; Thomas D Wang; Joseph C Liao Journal: J Endourol Date: 2009-02 Impact factor: 2.942
Authors: Matthias Trottmann; Ronald Sroka; Herbert Stepp; Bernhard Liedl; Armin J Becker; Christian G Stief; Sabine Kölle Journal: Lasers Med Sci Date: 2015-10-30 Impact factor: 3.161
Authors: Abhijit Mondal; John Lackey; Mossab Saeed; Fei-Yi Wu; Jordan K Johnson; Chao Huang; Frank B Sachse; Robert Hitchcock; Aditya K Kaza Journal: Semin Thorac Cardiovasc Surg Date: 2019-02-06
Authors: Zhixing Xie; Sung-Liang Chen; Mario L Fabiilli; J Brian Fowlkes; K Kirk Shung; Qifa Zhou; Paul L Carson; Xueding Wang Journal: Mol Imaging Date: 2013 Nov-Dec Impact factor: 4.488
Authors: Ryan G Koch; Alkiviadis Tsamis; Antonio D'Amore; William R Wagner; Simon C Watkins; Thomas G Gleason; David A Vorp Journal: J Biomech Date: 2014-01-20 Impact factor: 2.712
Authors: Timothy C Chang; Jen-Jane Liu; Shelly T Hsiao; Ying Pan; Kathleen E Mach; John T Leppert; Jesse K McKenney; Robert V Rouse; Joseph C Liao Journal: J Endourol Date: 2013-02-14 Impact factor: 2.942